Histone H3.3B Antibody

Novus Biologicals | Catalog # NBP3-48508

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Histone H3.3B Primary Antibodies »

Product Specifications

Immunogen

Full length human Histone H3.3B Recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H3.3B Antibody

Histone H3.3B Antibody

Immunohistochemistry-Paraffin: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H3.3B antibody (NBP3-48508) diluted at 1:500.
^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H3.3B Antibody

Immunohistochemistry-Paraffin: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H3.3B antibody (NBP3-48508) diluted at 1:500.
^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H3.3B Antibody

Immunohistochemistry: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H3.3B antibody (NBP3-48508) dilution: 1:500.


Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
Histone H3.3B Antibody

Immunohistochemistry: Histone H3.3B Antibody [NBP3-48508] -

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B(NBP3-48508) antibody at 1:500 dilution.

Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
Histone H3.3B Antibody

Immunohistochemistry: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse spinal cord by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
Histone H3.3B antibody (NBP3-48508) diluted at 1:500.


Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
Histone H3.3B Antibody

Immunohistochemistry-Paraffin: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H3.3B antibody (NBP3-48508) diluted at 1:500.
^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H3.3B Antibody

Western Blot: Histone H3.3B Antibody [NBP3-48508] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3B antibody (NBP3-48508) diluted at 1:10000.
Histone H3.3B Antibody

Immunohistochemistry: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse spinal cord by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
Histone H3.3B antibody (NBP3-48508) diluted at 1:500.

Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
Histone H3.3B Antibody

Immunocytochemistry/ Immunofluorescence: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3.3B protein stained by Histone H3.3B antibody (NBP3-48508) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Histone H3.3B Antibody

Western Blot: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects H3F3B protein by western blot analysis.
A. 50 ug rat brain lysate/extract
15% SDS-PAGE
Histone H3.3B antibody (NBP3-48508) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Histone H3.3B Antibody

Western Blot: Histone H3.3B Antibody [NBP3-48508] -

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
NBP3-48508 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Histone H3.3B Antibody

Immunohistochemistry-Paraffin: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Histone H3.3B antibody (NBP3-48508) diluted at 1:500.
^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H3.3B Antibody

Immunohistochemistry: Histone H3.3B Antibody [NBP3-48508] -

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H3.3B antibody (NBP3-48508) dilution: 1:500.

Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

Applications for Histone H3.3B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H3.3B

Histone H3.3B is 136 amino acids long, weighs approximately 15 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, malaria, leukemia, retinoblastoma, and immunodeficiency. Histone H3.3B has also been shown to have interactions with PELP1, HIST1H4A, HIST1H4B, HIST1H4C, and HIST1H4D in pathways such as the histone modification, development notch signaling, signal transduction for Activin A, amyloids, hemostasis, meiosis, meiotic recombination, and systemic lupus erythematosus pathways.

Alternate Names

H3 histone, family 3A, H3 histone, family 3B (H3.3B), H3.3BH3.3A, H3F3, H3F3A, histone H3.3

Gene Symbol

H3-3B

Additional Histone H3.3B Products

Product Documents for Histone H3.3B Antibody

Certificate of Analysis

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Product Specific Notices for Histone H3.3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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