Histone H4 [Trimethyl Lys20] Antibody - BSA Free

Novus Biologicals | Catalog # NB21-2090

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Mammal

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Dot Blot

Cited:

Western Blot, ICC/F

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone H4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic trimethylated peptide surrounding Lysine 20 of human Histone H4 [Swiss Prot# P62805].

Reactivity Notes

Predicted to react with most mammal species.

Modification

Trimethyl Lys20

Localization

Nucleus

Marker

H4K20me3

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H4 [Trimethyl Lys20] Antibody - BSA Free

Western Blot: Histone H4 [Trimethyl Lys20] AntibodyBSA Free [NB21-2090]

Western Blot: Histone H4 [Trimethyl Lys20] AntibodyBSA Free [NB21-2090]

Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.
Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in picomoles of peptide.
Simple Western: Histone H4 [Trimethyl Lys20] AntibodyBSA Free [NB21-2090]

Simple Western: Histone H4 [Trimethyl Lys20] AntibodyBSA Free [NB21-2090]

Simple Western: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Lane view shows a specific band for Histone H4 in 0.5 mg/ml of Hela Nuclear lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.

Applications for Histone H4 [Trimethyl Lys20] Antibody - BSA Free

Application
Recommended Usage

Dot Blot

0.5 ug/ml

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 23924899)

Immunohistochemistry

reported in scientific literature (PMID 23924899)

Simple Western

1:100

Western Blot

1:1000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hela Nuclear lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.58 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H4

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys16 (H4 K16Ac) plays a role in transcriptional activation and DNA repair. The protein MOF (MYST1) is the primary enzyme to acetylate K16, along with Gcn5, Esal, ATF2, and Sas2. Depletion of MOF reduces K16 acetylation, causing cell cycle arrests, chromosomal aberrations, and a poor DNA damage response.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4K20Me3

Entrez Gene IDs

121504 (Human); 320332 (Mouse)

Gene Symbol

H4-16

UniProt

P62805 (Human), P62806 (Mouse)

Additional Histone H4 Products

Product Documents for Histone H4 [Trimethyl Lys20] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H4 [Trimethyl Lys20] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Notch Pathway

Citations for Histone H4 [Trimethyl Lys20] Antibody - BSA Free

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail