Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Novus Biologicals | Catalog # NBP2-16848

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Dot Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Histone H4 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5/Lys8/Lys12/Lys16 of human Histone H4. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (100%).

Modification

ac Lys12, ac Lys16, ac Lys8, ac Lys5

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Histone-H4-[ac-Lys12--ac-Lys16--ac-Lys8--ac-Lys5]-Antibody-Western-Blot-NBP2-16848-img0015.jpg
Immunohistochemistry-Paraffin: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Immunohistochemistry-Paraffin: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-16848] - Human breast cancer, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Western Blot: Histone H4 Antibody [NBP2-16848] - WB for total histone in MCF-7 and HCT116 cells. Image from verified customer review.
Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 15% SDS PAGE diluted at 1:10000
Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:10000
Dot Blot: Histone H4 Antibody [NBP2-16848] - Peptide samples (0.1 ug) were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17) antibody Histone H4 at different dilution as indicated. A: Peptide samples of Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17). B: Peptide samples of Histone H4
Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Chronic gamma -radiation reduces histone levels. (a) Western blot analyses of histone H2AX & gamma H2AX in primary fibroblasts exposed to various dose-rates of chronic gamma -radiation for 7 days. Cells irradiated with a single acute dose of 4 Gy X-ray were included as control. (b) Effect of chronic gamma -irradiation on H2AX levels in three different isogenic primary cell types from a different donor to that used in (a) at the same dose rates as in (a). (c) Immunoblots of other histones in chronically irradiated primary fibroblasts. (d) All significant histone level changes detected by SILAC LC-MS/MS protein analyses of samples from primary fibroblasts exposed or mock-exposed to chronic gamma -radiation for 7 days. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate)
A: HeLa
15% SDS PAGE
NBP2-16848 diluted at 1:10000
Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody detects Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody (NBP2-16848) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.

Applications for Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Application
Recommended Usage

Dot Blot

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Brain, separated by Size, apparent MW was 11 kDa

Reviewed Applications

Read 1 review rated 5 using NBP2-16848 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone H4

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Epigenetics
Notch Pathway

Citations for Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Customer Reviews for Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 and HCT116 cells
    Species: Human
    Verified Customer | Posted 07/25/2014
    WB for total hsitoen in MCF-7 and HCT116 cells
    Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody NBP2-16848

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail