HIV-1 Rev binding protein Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91991

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HIV-1 Rev binding protein Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant HIV-1 Rev binding protein corresponding to amino acids: PQSTATANFANFAHFNSHAAQNSANADFANFDAFGQSSGSSNFGGFPTASHSPFQPQTTGGSAASVNANFAHFDNFPKSSSADFGTFNTSQSHQTASAVSKVSTNKAGLQTADKYAALANLDNIFSAG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIV-1 Rev binding protein Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HIV-1 Rev binding protein Antibody [NBP1-91991]

Immunocytochemistry/ Immunofluorescence: HIV-1 Rev binding protein Antibody [NBP1-91991]

Immunocytochemistry/Immunofluorescence: HIV-1 Rev binding protein Antibody [NBP1-91991] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: HIV-1 Rev binding protein Antibody [NBP1-91991]

Immunohistochemistry-Paraffin: HIV-1 Rev binding protein Antibody [NBP1-91991]

Immunohistochemistry-Paraffin: HIV-1 Rev binding protein Antibody [NBP1-91991] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Western Blot: HIV-1 Rev binding protein Antibody [NBP1-91991]

Western Blot: HIV-1 Rev binding protein Antibody [NBP1-91991]

Western Blot: HIV-1 Rev binding protein Antibody [NBP1-91991] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
HIV-1 Rev binding protein Antibody - BSA Free Western Blot: HIV-1 Rev binding protein Antibody - BSA Free [NBP1-91991]

Western Blot: HIV-1 Rev binding protein Antibody - BSA Free [NBP1-91991]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
HIV-1 Rev binding protein Antibody - BSA Free Western Blot: HIV-1 Rev binding protein Antibody - BSA Free [NBP1-91991]

Western Blot: HIV-1 Rev binding protein Antibody - BSA Free [NBP1-91991]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Applications for HIV-1 Rev binding protein Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIV-1 Rev binding protein

HIV-1 Rev binding protein (HRB) is encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ArfGAP with FG repeats 1, DKFZp686I15205, HIV-1 Rev-binding protein, HRBRev interacting protein, Rab, Rev/Rex activation domain-binding protein, RABMGC116938, RIPMGC116940

Gene Symbol

AGFG1

Additional HIV-1 Rev binding protein Products

Product Documents for HIV-1 Rev binding protein Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HIV-1 Rev binding protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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