HJURP Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-47287
![Western Blot: HJURP Antibody [NBP1-47287]](https://resources.rndsystems.com/images/products/HJURP-Antibody-Western-Blot-NBP1-47287-img0004.jpg "Western Blot: HJURP Antibody [NBP1-47287]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 650 and 700 of human Holliday Junction Recognition Protein using the numbering given in entry NP_060880.3 (GeneID 55355).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HJURP Antibody - BSA Free
Western Blot: HJURP Antibody [NBP1-47287]
Western Blot: HJURP Antibody [NBP1-47287] - Detection of human HJURP by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HJURP antibody NBP1-47287 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HJURP was also immunoprecipitated by rabbit anti-HJURP antibody NBP1-472878, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).![HJURP Antibody [NBP1-47287]](https://resources.rndsystems.com/images/products/antibody/nbp1-47287_rabbit-polyclonal-hjurp-antibody-immunohistochemistry-paraffin-184202372737.jpg "HJURP Antibody [NBP1-47287]-Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HJURP used at a dilution of 1:5,000 (0.2µg/ml) Detection: DAB")
HJURP Antibody [NBP1-47287]-Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HJURP used at a dilution of 1:5,000 (0.2µg/ml) Detection: DAB
HJURP Antibody [NBP1-47287]-Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HJURP used at a dilution of 1:5,000 (0.2µg/ml) Detection: DAB![HJURP Antibody [NBP1-47287]](https://resources.rndsystems.com/images/products/antibody/nbp1-47287_rabbit-polyclonal-hjurp-antibody-western-blot-184202372927.jpg "HJURP Antibody [NBP1-47287]- Whole cell lysate (2 µg) from K-562, U2OS, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HJURP antibody used for WB at 0.04 µg/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.")
HJURP Antibody [NBP1-47287]- Whole cell lysate (2 µg) from K-562, U2OS, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HJURP antibody used for WB at 0.04 µg/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.
HJURP Antibody [NBP1-47287]- Whole cell lysate (2 µg) from K-562, U2OS, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HJURP antibody used for WB at 0.04 µg/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.Applications for HJURP Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Immunoprecipitation
2 -10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HJURP
Alternate Names
FAKTS14-3-3-associated AKT substrate, fetal liver expressing gene 1, Fetal liver-expressing gene 1 protein, FLEG1, hFLEG1, Holliday junction recognition protein, Up-regulated in lung cancer 9, URLC9DKFZp762E1312
Entrez Gene IDs
55355 (Human)
Gene Symbol
HJURP
Additional HJURP Products
Product Documents for HJURP Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HJURP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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