HLA B Antibody (2G7B10) - BSA Free

Novus Biologicals | Catalog # NBP2-61871

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2G7B10

Format

BSA Free
View all HLA-B Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human HLA B (AA: 241-362) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HLA B Antibody (2G7B10) - BSA Free

Western Blot: HLA B Antibody (2G7B10)BSA Free [NBP2-61871]

Western Blot: HLA B Antibody (2G7B10)BSA Free [NBP2-61871]

Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Immunocytochemistry/ Immunofluorescence: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of bladder cancer tissues using HLA-B mouse mAb with DAB staining.
Flow Cytometry: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Flow Cytometry: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Flow Cytometry: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of MCF-7 cells using HLA-B mouse mAb (green) and negative control (red).
Western Blot: HLA B Antibody (2G7B10)BSA Free [NBP2-61871]

Western Blot: HLA B Antibody (2G7B10)BSA Free [NBP2-61871]

Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against human HLA-B (AA: 241-362) recombinant protein. (Expected MW is 38.4 kDa)
Western Blot: HLA B Antibody (2G7B10)BSA Free [NBP2-61871]

Western Blot: HLA B Antibody (2G7B10)BSA Free [NBP2-61871]

Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mouse mAb against Ramos (1) and A431 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Immunocytochemistry/ Immunofluorescence: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of ovarian cancer tissues using HLA-B mouse mAb with DAB staining.
ELISA: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

ELISA: HLA B Antibody (2G7B10) - BSA Free [NBP2-61871]

ELISA: HLA B Antibody (2G7B10) [NBP2-61871] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for HLA B Antibody (2G7B10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA-B

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.;

Long Name

Major Histocompatibility Complex, Class I, B

Alternate Names

B-4901, HLAB

Gene Symbol

HLA-B

Additional HLA-B Products

Product Documents for HLA B Antibody (2G7B10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HLA B Antibody (2G7B10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

Customer Reviews for HLA B Antibody (2G7B10) - BSA Free

There are currently no reviews for this product. Be the first to review HLA B Antibody (2G7B10) - BSA Free and earn rewards!

Have you used HLA B Antibody (2G7B10) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies