HLA DRB1 Antibody (SPM288)

Novus Biologicals | Catalog # NBP2-45312

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Monkey, Mouse (Negative)

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SPM288
View all HLA-DRB1 Primary Antibodies »

Product Specifications

Immunogen

Activated human peripheral blood mononuclear cells

Reactivity Notes

Does not react with Mouse.

Localization

Cell Surface

Specificity

This monoclonal antibody reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11419)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for HLA DRB1 Antibody (SPM288)

Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM288).
Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DRB1 Antibody (SPM288).
Flow Cytometry: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Flow Cytometry: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Flow Cytometry: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (SPM288).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (SPM288).
Immunofluorescence: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Immunofluorescence: HLA DRB1 Antibody (SPM288) [NBP2-45312]

Immunofluorescence: HLA DRB1 Antibody (SPM288) [NBP2-45312] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with APC (Green). The nuclear counterstain is Red Dot (Red)

Applications for HLA DRB1 Antibody (SPM288)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HLA-DRB1

HLA DRB1 can be used for HLA typing.

Long Name

Major histocompatibility complex, class II, DR beta 1

Alternate Names

DRB1, HLA-DR1B, HLA-DRB, SS1

Gene Symbol

HLA-DRB1

UniProt

P01911 (Human)

Additional HLA-DRB1 Products

Product Documents for HLA DRB1 Antibody (SPM288)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HLA DRB1 Antibody (SPM288)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HLA DRB1 Antibody (SPM288)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HLA DRB1 Antibody (SPM288)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does the HLA DRB1 mAb SPM288 react with all DRB1 alleles? Also, is this product purified IgG?

    A: It looks like this antibody was not specifically tested against all alleles. All we know is that it reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ.

  • Q: We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?

    A:

    While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

  • Q: Does the HLA DRB1 mAb SPM288 react with all DRB1 alleles? Also, is this product purified IgG?

    A: It looks like this antibody was not specifically tested against all alleles. All we know is that it reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ.

  • Q: We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?

    A:

    While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

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