HMG-CoA Reductase/HMGCR Antibody (CL0259) - BSA Free

Novus Biologicals | Catalog # NBP2-61616

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0259

Format

BSA Free
View all HMG-CoA Reductase/HMGCR Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P04035, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for HMG-CoA Reductase/HMGCR Antibody (CL0259) - BSA Free

Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HMGCR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line MCF-7 Lane 3: Human cell line HepG2
Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human liver shows strong cytoplasmic immunoreactivity.
Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human breast cancer shows moderate cytoplasmic immunoreactivity in tumor cells.
Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human cervix shows cytoplasmic immunoreactivity in epithelial cells.
Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human liver cancer shows strong cytoplasmic positivity in tumor cells.

Applications for HMG-CoA Reductase/HMGCR Antibody (CL0259) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMG-CoA Reductase/HMGCR

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

3-Hydroxy-3-Methylglutaryl-CoA Reductase

Alternate Names

HMGCR, Hydroxymethylglutaryl-CoA Reductase, LDLCQ3

Gene Symbol

HMGCR

Additional HMG-CoA Reductase/HMGCR Products

Product Documents for HMG-CoA Reductase/HMGCR Antibody (CL0259) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HMG-CoA Reductase/HMGCR Antibody (CL0259) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for HMG-CoA Reductase/HMGCR Antibody (CL0259) - BSA Free

Customer Reviews for HMG-CoA Reductase/HMGCR Antibody (CL0259) - BSA Free

There are currently no reviews for this product. Be the first to review HMG-CoA Reductase/HMGCR Antibody (CL0259) - BSA Free and earn rewards!

Have you used HMG-CoA Reductase/HMGCR Antibody (CL0259) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies