HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43933

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0260

Format

Azide and BSA Free
View all HMG-CoA Reductase/HMGCR Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P04035, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free

HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Analysis in human liver and skeletal muscle tissues using NBP3-43933 antibody. Corresponding HMG-CoA Reductase/HMGCR RNA-seq data are presented for the same tissues.
HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Staining of human breast cancer shows moderate to strong cytoplasmic positivity in tumor cells.
HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Staining of human liver cancer shows strong cytoplasmic positivity in tumor cells.
HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free Western Blot: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Western Blot: HMG-CoA Reductase/HMGCR Antibody [NBP3-43933]

Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HMG-CoA Reductase/HMGCR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HMG-CoA Reductase/HMGCR

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

3-Hydroxy-3-Methylglutaryl-CoA Reductase

Alternate Names

HMGCR, Hydroxymethylglutaryl-CoA Reductase, LDLCQ3

Gene Symbol

HMGCR

Additional HMG-CoA Reductase/HMGCR Products

Product Documents for HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HMG-CoA Reductase/HMGCR Antibody (CL0260) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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