HMGA2 Antibody (763) - Azide and BSA Free

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640]

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - RT2 xenograft. HMGA2 antibody [763] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640]

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - SkHep1 xenograft. HMGA2 antibody [763] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640]

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - Rat heart. HMGA2 stained by HMGA2 antibody [763] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation: HMGA2 Antibody (763) [NBP2-43640]

Immunoprecipitation: HMGA2 Antibody (763) [NBP2-43640] - Analysis of HMGA2 protein from HepG2 nuclear extracts using 5 ug of HMGA2 antibody [763]. Western blot analysis was performed using HMGA2 antibody [763]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: HMGA2 Antibody (763) [NBP2-43640] -

Western Blot: HMGA2 Antibody (763) [NBP2-43640] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: HMGA2 Antibody (763) [NBP2-43640] -

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (NBP2-43640) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: HMGA2 Antibody (763) [NBP2-43640] -

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (NBP2-43640) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: HMGA2 Antibody (763) [NBP2-43640] -

HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGA2 protein stained by HMGA2 antibody [GT763] (NBP2-43640) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Western Blot: HMGA2 Antibody (763) - Azide and BSA Free [NBP2-43640] -

EZH2/HMGA2 regulates the PI3K/AKT signaling. MI rats were subjected to injection of EZH2-OE or EZH2-NC (MI + MSC-EXO + EZH2 NC/EZH2 OE) and EZH2-OE + HMGA2-OE or EZH2-OE + HMGA2-NC. A HMGA2 protein expression in myocardial tissues in response to EZH2-OE + HMGA2-OE examined using western blot (Additional file 5: Fig. S5). B Weight change in rats after EZH2-OE + HMGA2-OE treatment. C Changes in HMI in rats after EZH2-OE + HMGA2-OE treatment. D Measurement of Dd, Sd, and LVEF by echocardiography at 2 weeks after LAD in rats. E Measurement of LVMI by echocardiography in rats. F KEGG pathway analysis of gene enrichment pathways [29]. G Changes in PI3K/AKT pathway in rat myocardium examined using western blot (Additional file 6: Fig. S6). All data are expressed as mean +/- SD (n = 6/group, *p < 0.05 vs. Sham, DMSO, MI + MSC-EXO + EZH2-NC or EZH2-OE + HMGA2-NC group determined by unpaired t test or one-way ANOVA) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35264108), licensed under a CC-BY license. Not internally tested by Novus Biologicals.