HMGCL Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32767

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HMGCL Primary Antibodies »

Product Specifications

Immunogen

Full length human HMGCL Recombinant protein.

Reactivity Notes

Bovine (88%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMGCL Antibody - BSA Free

Western Blot: HMGCL Antibody [NBP1-32767]

Western Blot: HMGCL Antibody [NBP1-32767]

Western Blot: HMGCL Antibody [NBP1-32767] - rat liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:10000.
Immunocytochemistry/ Immunofluorescence: HMGCL Antibody [NBP1-32767]

Immunocytochemistry/ Immunofluorescence: HMGCL Antibody [NBP1-32767]

Immunocytochemistry/Immunofluorescence: HMGCL Antibody [NBP1-32767] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HMGCL protein stained by HMGCL antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-32767]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-32767]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-32767] - SW480 xenograft, using HMGCL antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: HMGCL Antibody [NBP1-32767]

Western Blot: HMGCL Antibody [NBP1-32767]

Western Blot: HMGCL Antibody [NBP1-32767] - (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:3000
Western Blot: HMGCL Antibody [NBP1-32767]

Western Blot: HMGCL Antibody [NBP1-32767]

Western Blot: HMGCL Antibody [NBP1-32767] - mouse liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:10000.

Applications for HMGCL Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HMGCL

The protein encoded by the HMGCL gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes thefinal step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associatedwith HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been foundfor this gene. (provided by RefSeq)

Alternate Names

3-hydroxy-3-methylglutaryl-CoA lyase, 3-hydroxymethyl-3-methylglutaryl-CoA lyase, EC 4.1.3.4, HLmitochondrial

Gene Symbol

HMGCL

Additional HMGCL Products

Product Documents for HMGCL Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HMGCL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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