![Western Blot: HMGN3/TRIP7 Antibody [NB100-59840]](https://resources.rndsystems.com/images/products/HMGN3-TRIP7-Antibody-Western-Blot-NB100-59840-img0003.jpg "Western Blot: HMGN3/TRIP7 Antibody [NB100-59840]")
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 60 and 99 of human high mobility group nucleosomal binding domain 3 using the numbering given in entry NP_004233.1 (GeneID 9324).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HMGN3/TRIP7 Antibody
Western Blot: HMGN3/TRIP7 Antibody [NB100-59840]
Western Blot: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HMGN3 antibody NB100-59840 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry: HMGN3/TRIP7 Antibody [NB100-59840]](https://resources.rndsystems.com/images/products/HMGN3-TRIP7-Antibody-Immunohistochemistry-NB100-59840-img0004.jpg "Immunohistochemistry: HMGN3/TRIP7 Antibody [NB100-59840]")
Immunohistochemistry: HMGN3/TRIP7 Antibody [NB100-59840]
Immunohistochemistry: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HMGN3 (NB100-59840). Detection: DAB![Immunoprecipitation: HMGN3/TRIP7 Antibody [NB100-59840]](https://resources.rndsystems.com/images/products/HMGN3-TRIP7-Antibody-Immunoprecipitation-NB100-59840-img0005.jpg "Immunoprecipitation: HMGN3/TRIP7 Antibody [NB100-59840]")
Immunoprecipitation: HMGN3/TRIP7 Antibody [NB100-59840]
Immunoprecipitation: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGN3 antibody NB100-59840 (lot 2) used for IP at 6 ug per reaction. HMGN3 was also immunoprecipitated with a previous lot of this antibody (lot 1) and rabbit anti-HMGN3 antibody NB100-59839 (lots 1 and 2). For blotting immunoprecipitated HGMN3, NB100-59840 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for HMGN3/TRIP7 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000
Immunoprecipitation
2-5ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HMGN3/TRIP7
Alternate Names
DKFZp686E20226, high mobility group nucleosomal binding domain 3, high mobility group nucleosome-binding domain-containing protein 3, high-mobility group protein HMGN3, PNAS-25, thyroid hormone receptor interacting protein 7, thyroid hormone receptor interactor 7, Thyroid receptor-interacting protein 7, TR-interacting protein 7, TRIP-7, TRIP7FLJ42187
Entrez Gene IDs
9324 (Human)
Gene Symbol
HMGN3
UniProt
Additional HMGN3/TRIP7 Products
Product Documents for HMGN3/TRIP7 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HMGN3/TRIP7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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