HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free

Novus Biologicals | Catalog # NBP3-26572

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3B2 expressed in HEK293

Format

BSA Free
View all HNF-3 alpha/FoxA1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human HNF-3 alpha/FoxA1 [UniProt P55317]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free

HNF-3 alpha/FoxA1 Antibody (3B2)

Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] -

Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Immunoprecipitating HNF-3 alpha/FoxA1 in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26572 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26572 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
HNF-3 alpha/FoxA1 Antibody (3B2)

Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] -

Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Image of HNF-3 alpha/FoxA1 Antibody (3B2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
HNF-3 alpha/FoxA1 Antibody (3B2)

Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] -

Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Image of HNF-3 alpha/FoxA1 Antibody (3B2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
HNF-3 alpha/FoxA1 Antibody (3B2)

Western Blot: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] -

Western Blot: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Positive Western Blot detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate.
All lanes: HNF-3 alpha/FoxA1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 50, 46 kDa
Observed band size: 50 kDa

Applications for HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: HNF-3 alpha/FoxA1

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1

Alternate Names

FoxA1, HNF3 alpha, TCF3A

Gene Symbol

FOXA1

Additional HNF-3 alpha/FoxA1 Products

Product Documents for HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free

There are currently no reviews for this product. Be the first to review HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free and earn rewards!

Have you used HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies