HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-45355
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (98%), Rat (96%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 3B3NB
Format
Azide and BSA Free
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Product Specifications
Immunogen
Partial recombinant human FOXA1 protein (between amino acids 350-473) [UniProt# P55317].
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (78%)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free
![Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB)Azide and BSA Free [NBP2-45355]](https://resources.rndsystems.com/images/products/HNF-3-alpha-FoxA1-Antibody-3B3NB-Azide-Free-Western-Blot-NBP2-45355-img0009.jpg "Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB)Azide and BSA Free [NBP2-45355]")
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB)Azide and BSA Free [NBP2-45355]
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Analysis of FOXA1 (3B3NB) in partial recombinant FOXA1 protein and PC3 lysate, respectivetly. Image using the standard format of this product.![Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]](https://resources.rndsystems.com/images/products/HNF-3-alpha-FoxA1-Antibody-3B3NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45355-img0005.jpg "Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]")
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]
Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - FOXA1 (3B3NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respective. Image using the standard format of this product.![Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]](https://resources.rndsystems.com/images/products/HNF-3-alpha-FoxA1-Antibody-3B3NB-Azide-Free-Immunohistochemistry-NBP2-45355-img0007.jpg "Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]")
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Analysis of FoxA1 in human prostate. Image using the standard format of this product.![Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]](https://resources.rndsystems.com/images/products/HNF-3-alpha-FoxA1-Antibody-3B3NB-Azide-Free-Flow-Cytometry-NBP2-45355-img0004.jpg "Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]")
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Staining of LnCAP cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with the negative control (blue).![Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB)Azide and BSA Free [NBP2-45355]](https://resources.rndsystems.com/images/products/HNF-3-alpha-FoxA1-Antibody-3B3NB-Azide-Free-Western-Blot-NBP2-45355-img0008.jpg "Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB)Azide and BSA Free [NBP2-45355]")
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB)Azide and BSA Free [NBP2-45355]
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - (1) Whole cell protein from PC3 and (2) MCF7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4 ug/ml anti-hFOXA1 (3B3NB) in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image using the standard format of this product.![Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]](https://resources.rndsystems.com/images/products/HNF-3-alpha-FoxA1-Antibody-3B3NB-Azide-Free-Immunohistochemistry-NBP2-45355-img0006.jpg "Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]")
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Analysis of FoxA1 in human breast adenocarcinoma. Image using the standard format of this product.![Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]](https://resources.rndsystems.com/images/products/HNF-3-alpha-FoxA1-Antibody-3B3NB-Azide-Free-Flow-Cytometry-NBP2-45355-img0002.jpg "Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]")
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free [NBP2-45355]
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Staining of HepG2 cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with the negative control (blue).Applications for HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
25 ug/ml
Immunocytochemistry/ Immunofluorescence
1:25 - 1:50
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
2-4 ug/ml
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HNF-3 alpha/FoxA1
Long Name
Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1
Alternate Names
FoxA1, HNF3 alpha, TCF3A
Gene Symbol
FOXA1
Additional HNF-3 alpha/FoxA1 Products
Product Documents for HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
