HNF-4 alpha/NR2A1 Antibody

Novus Biologicals | Catalog # NB100-1783

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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Mouse, Primate

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HNF-4 alpha/NR2A1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence RLSKTLVDMDMADY-C corresponding to N-Terminus according to NP_849180.1, NP_000448.3, NP_849181.1.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID18561282).

Specificity

This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HNF-4 alpha/NR2A1 Antibody

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NB100-1783]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NB100-1783]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NB100-1783] - Staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: HNF-4 alpha/NR2A1 Antibody [NB100-1783]

Western Blot: HNF-4 alpha/NR2A1 Antibody [NB100-1783]

Western Blot: HNF-4 alpha/NR2A1 Antibody [NB100-1783] - (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for HNF-4 alpha/NR2A1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1-0.5 ug/ml
Application Notes
WB: Approx 55kDa and 48kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3, 46.6kDa according to Human NP_849181.1). Primary incubation was 1 hour.

Reviewed Applications

Read 1 review rated 1 using NB100-1783 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

UniProt

NP_849180.1 (Human)

Additional HNF-4 alpha/NR2A1 Products

Product Documents for HNF-4 alpha/NR2A1 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for HNF-4 alpha/NR2A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HNF-4 alpha/NR2A1 Antibody

Customer Reviews for HNF-4 alpha/NR2A1 Antibody (1)

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Customer Images

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  • HNF-4 alpha/NR2A1 Antibody
    Name: Marisa Ferreira
    Application: Immunocytochemistry
    Sample Tested: Mouse Liver Tissue
    Species: Mouse
    Verified Customer | Posted 06/29/2023
    FFPE, Tris-EDTA: A - DAPI; B - HNF-4 alpha alternative antibody, 488 laser; C - NB100-1783 antibody, 555 laser.
    HNF-4 alpha/NR2A1 Antibody NB100-1783
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail