HNF-4 alpha/NR2A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89679

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HNF-4 alpha/NR2A1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HNF-4 alpha/NR2A1 Antibody - BSA Free

HNF-4 alpha/NR2A1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Immunocytochemistry/ Immunofluorescence: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Staining of human cell line CACO-2 shows localization to nucleoplasm.
HNF-4 alpha/NR2A1 Antibody - BSA Free Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Analysis in human duodenum and tonsil tissues using NBP1-89679 antibody. Corresponding HNF4A RNA-seq data are presented for the same tissues.
HNF-4 alpha/NR2A1 Antibody - BSA Free Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Staining of human duodenum shows strong nuclear positivity in glandular cells.
HNF-4 alpha/NR2A1 Antibody - BSA Free Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Staining of human tonsil shows no positivity as expected.
HNF-4 alpha/NR2A1 Antibody - BSA Free Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Staining of human kidney shows moderate nuclear positivity in cells in tubules.
HNF-4 alpha/NR2A1 Antibody - BSA Free Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.
HNF-4 alpha/NR2A1 Antibody - BSA Free Western Blot: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Western Blot: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Analysis in human cell lines Caco-2 and PC-3 using Anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
HNF-4 alpha/NR2A1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

Chromatin Immunoprecipitation-exo-Seq: HNF-4 alpha/NR2A1 Antibody - BSA Free [NBP1-89679]

ChIP-Exo-Seq composite graph for Anti-HNF4A (NBP1-89679) tested in HepG2 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for HNF-4 alpha/NR2A1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-89679 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

HNF4 alpha is encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

UniProt

P41235

Additional HNF-4 alpha/NR2A1 Products

Product Documents for HNF-4 alpha/NR2A1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HNF-4 alpha/NR2A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HNF-4 alpha/NR2A1 Antibody - BSA Free

Customer Reviews for HNF-4 alpha/NR2A1 Antibody - BSA Free (1)

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Customer Images

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  • HNF-4 alpha/NR2A1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen Rat Liver Tissue sections
    Species: Rat
    Verified Customer | Posted 11/19/2019
    HNF4a green, myeloperoxidase red, DAPI blue
    8um section fixed in formalin HNF4a antibody diluted 1:100 Multiplexed with myeloperoxidase antibody
    HNF-4 alpha/NR2A1 Antibody - BSA Free NBP1-89679

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail