![Western Blot: HNF-6/ONECUT1 Antibody [NBP2-27079]](https://resources.rndsystems.com/images/products/HNF-6-ONECUT1-Antibody-Western-Blot-NBP2-27079-img0004.jpg "Western Blot: HNF-6/ONECUT1 Antibody [NBP2-27079]")
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Key Product Details
Species Reactivity
Validated:
Human, Canine, Primate
Predicted:
Bovine (94%), Chimpanzee (100%), Monkey (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
A portion of amino acids 420-465 of human HNF6 was used as the immunogen.
Reactivity Notes
The amino acid sequence used as immunogen is 88% homologous in Mouse and Opossum, and 82% homologous in Zebrafish.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HNF-6/ONECUT1 Antibody
Western Blot: HNF-6/ONECUT1 Antibody [NBP2-27079]
Western Blot: HNF-6/ONECUT1 Antibody [NBP2-27079] - Analysis ofHNF6 inhuman pancreas in the 1) absence and 2) presence of immunizing peptide, and 3) rat pancreasusing this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.![Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-27079]](https://resources.rndsystems.com/images/products/HNF-6-ONECUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-27079-img0003.jpg "Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-27079]")
Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-27079]
Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-27079] - Analysis of HNF6 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.Applications for HNF-6/ONECUT1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5ug/ml
Western Blot
3-5ug/ml~
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HNF-6/ONECUT1
Long Name
Hepatocyte Nuclear Factor 6
Alternate Names
HNF-6A, HNF6, OC-1, ONECUT1
Gene Symbol
ONECUT1
UniProt
Additional HNF-6/ONECUT1 Products
Product Documents for HNF-6/ONECUT1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HNF-6/ONECUT1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
