hnRNP AB Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91999

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all hnRNP AB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSEAGEEQPMETTGATENGHEAV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP AB Antibody - BSA Free

Western Blot: hnRNP AB Antibody [NBP1-91999]

Western Blot: hnRNP AB Antibody [NBP1-91999]

Western Blot: hnRNP AB Antibody [NBP1-91999] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: hnRNP AB Antibody [NBP1-91999]

Immunocytochemistry/ Immunofluorescence: hnRNP AB Antibody [NBP1-91999]

Immunocytochemistry/Immunofluorescence: hnRNP AB Antibody [NBP1-91999] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: hnRNP AB Antibody [NBP1-91999]

Immunohistochemistry-Paraffin: hnRNP AB Antibody [NBP1-91999]

Immunohistochemistry-Paraffin: hnRNP AB Antibody [NBP1-91999] - Staining of human uterus, post-menopause shows strong nuclear positivity in glandular cells.
hnRNP AB Antibody - BSA Free Western Blot: hnRNP AB Antibody - BSA Free [NBP1-91999]

Western Blot: hnRNP AB Antibody - BSA Free [NBP1-91999]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
hnRNP AB Antibody - BSA Free Western Blot: hnRNP AB Antibody - BSA Free [NBP1-91999]

Western Blot: hnRNP AB Antibody - BSA Free [NBP1-91999]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for hnRNP AB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP AB

hnRNP AB belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Alternate Names

catalytic polypeptide 1-binding protein 1, heterogeneous nuclear ribonucleoprotein A/B, hnRNP A/B, hnRNP type A/B protein

Gene Symbol

HNRNPAB

Additional hnRNP AB Products

Product Documents for hnRNP AB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for hnRNP AB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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