hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44054

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL2593

Format

Azide and BSA Free
View all hnRNP C1 + C2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P07910, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free

hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining in U2OS cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining in U251 cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Western Blot: hnRNP C1 + C2 Antibody [NBP3-44054]

Western Blot: hnRNP C1 + C2 Antibody [NBP3-44054]

Lane 1: Marker [kDa]
Lane 2:Human cell line U-251 MG
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining in MCF7 cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining in HeLa cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining in A431 cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining of human fallopian tube shows strong nuclear immunoreactivity.
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining of human kidney shows strong nuclear immunoreactivity.
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining of human prostate shows strong nuclear positivity in glandular and connective tissue cells.
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining of human rectum shows strong nuclear immunoreactivity in glandular and lamina propria cells.
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining of human small intestine shows strong nuclear positivity in glandular epithelium and in lamina propria cells.
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP3-44054]

Staining of human testis shows nuclear positivity in seminiferous tubules.
hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Western Blot: hnRNP C1 + C2 Antibody [NBP3-44054]

Western Blot: hnRNP C1 + C2 Antibody [NBP3-44054]

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-hnRNP C1 + C2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Applications for hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, Fixation/Permeabilization with PFA/Triton X-100 is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: hnRNP C1 + C2

RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. When this is performed, the hnRNPs are isolated (consisting of protein groups named A to U), and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weight starting with 34 kDa up to 43 kDa. The hnRNP-C proteins have a single RNP motif RNA-binding domain (RBD) of 80 to 100 amino acid long. The hnRNP -C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding.4 Although its physiological action is unknown, mutation of the hnRNPC gene causes a embryonic lethal phenotype.

Alternate Names

C1, C2, heterogeneous nuclear ribonucleoprotein C (C1/C2), heterogeneous nuclear ribonucleoproteins C1/C2, HNRNP, hnRNP C1/C2, hnRNPC, HNRPC, MGC104306, MGC105117, MGC117353, MGC131677, nuclear ribonucleoprotein particle C1 protein, nuclear ribonucleoprotein particle C2 protein, SNRPC

Gene Symbol

HNRNPC

Additional hnRNP C1 + C2 Products

Product Documents for hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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