hnRNP F Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33607

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (97%), Chimpanzee (100%), Rat (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all hnRNP F Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP F. The exact sequence is proprietary.

Localization

Nucleus, nucleoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for hnRNP F Antibody - BSA Free

Western Blot: hnRNP F Antibody [NBP1-33607]

Western Blot: hnRNP F Antibody [NBP1-33607]

Western Blot: hnRNP F Antibody [NBP1-33607] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: hnRNP F Antibody [NBP1-33607]

Immunocytochemistry/ Immunofluorescence: hnRNP F Antibody [NBP1-33607]

Immunocytochemistry/Immunofluorescence: hnRNP F Antibody [NBP1-33607] - Paraformaldehyde-fixed A431, using hnRNP F (NBP1-33607) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607]

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607]

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607] - Mouse uterus. hnRNP F antibody [N1N3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: hnRNP F Antibody [NBP1-33607]

Western Blot: hnRNP F Antibody [NBP1-33607]

Western Blot: hnRNP F Antibody [NBP1-33607] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607]

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607]

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607] - A549 Xenograft, using hnRNP F antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: hnRNP F Antibody [NBP1-33607]

Immunoprecipitation: hnRNP F Antibody [NBP1-33607]

Immunoprecipitation: hnRNP F Antibody [NBP1-33607] - Immunoprecipitation of hnRNP F protein from 293T whole cell extracts using 5 ug of hnRNP F antibody [N1N3]. Western blot analysis was performed using hnRNP F antibody [N1N3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Applications for hnRNP F Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Knockdown Validated

Validation (PMID: 31814907).

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: hnRNP F

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and in particular, the hnRNP A1 protein is one of the major premRNA/ mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, including hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the premRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines.

Alternate Names

heterogeneous nuclear ribonucleoprotein F, hnRNP F, HNRPFHnRNP F protein, mcs94-1, MGC110997, Nucleolin-like protein mcs94-1, OK/SW-cl.23

Gene Symbol

HNRNPF

Additional hnRNP F Products

Product Documents for hnRNP F Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for hnRNP F Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for hnRNP F Antibody - BSA Free

Customer Reviews for hnRNP F Antibody - BSA Free

There are currently no reviews for this product. Be the first to review hnRNP F Antibody - BSA Free and earn rewards!

Have you used hnRNP F Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies