hnRNP F Antibody - BSA Free

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-57273]

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-57273] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.

Immunocytochemistry/immunofluorescence: hnRNP F Antibody [NBP1-57273] -

nbp1-57273_rabbit-polyclonal-hnrnp-f-antibody-20920231742122.jpg

Western Blot: hnRNP F Antibody [NBP1-57273] -

Western Blot: hnRNP F Antibody [NBP1-57273] - SMN, PLS3, hnRNP F, & hnRNP H1/2 associate in a large complex. a SMN & PLS3 independently associated with hnRNP H1/2 based on co-immunoprecipitation from HEK293T cells transfected with tagged proteins. b Endogenous PLS3, hnRNP F, & hnRNP H 1/2 associated with SMN in mouse brain extracts, based on immunoprecipitation with antisera against endogenous SMN protein. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. c Endogenous SMN, hnRNP F, & hnRNP H1/2 associated with endogenous PLS3 using the same approach outlined in b. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. IP on endogenous mouse brain extract was done from 3 independent brain extractions & preparations. d Size fractionation studies of whole cell lysates from HEK293T cells showed that actin, SMN, PLS3, & hnRNP F and/or H1/2 proteins co-fractionate in complexes of the indicated size (bracket), although the bulk of hnRNP F/H1/2 protein is in other smaller complexes. HEK293T cells were transfected with PLS3-Flag, & hnRNP F-GFP, endogenous hnRNP H1/2, actin, & SMN were detected using antisera Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32938453), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: hnRNP F Antibody [NBP1-57273] -

Western Blot: hnRNP F Antibody [NBP1-57273] - SMN, PLS3, hnRNP F, & hnRNP H1/2 associate in a large complex. a SMN & PLS3 independently associated with hnRNP H1/2 based on co-immunoprecipitation from HEK293T cells transfected with tagged proteins. b Endogenous PLS3, hnRNP F, & hnRNP H 1/2 associated with SMN in mouse brain extracts, based on immunoprecipitation with antisera against endogenous SMN protein. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. c Endogenous SMN, hnRNP F, & hnRNP H1/2 associated with endogenous PLS3 using the same approach outlined in b. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. IP on endogenous mouse brain extract was done from 3 independent brain extractions & preparations. d Size fractionation studies of whole cell lysates from HEK293T cells showed that actin, SMN, PLS3, & hnRNP F and/or H1/2 proteins co-fractionate in complexes of the indicated size (bracket), although the bulk of hnRNP F/H1/2 protein is in other smaller complexes. HEK293T cells were transfected with PLS3-Flag, & hnRNP F-GFP, endogenous hnRNP H1/2, actin, & SMN were detected using antisera Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32938453), licensed under a CC-BY license. Not internally tested by Novus Biologicals.