hnRNP G Antibody (SR2422)

Name: hnRNP G Antibody (SR2422)
Brand: Novus Biologicals
SKU: NBP3-27724
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-27724

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2422 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide of human hnRNP G (Uniprot #: P38159)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for hnRNP G Antibody (SR2422)

Western Blot: hnRNP G Antibody (SR2422) [NBP3-27724] -

Western Blot: hnRNP G Antibody (SR2422) [NBP3-27724] - Western blot analysis of hnRNP G in K562, Hela lysates

Immunocytochemistry/ Immunofluorescence: hnRNP G Antibody (SR2422) [NBP3-27724] -

Immunocytochemistry/ Immunofluorescence: hnRNP G Antibody (SR2422) [NBP3-27724] - Immunocytochemistry analysis of hnRNP G (green) in 293T using NBP3-27724, and DAPI (blue).

Immunohistochemistry-Paraffin: hnRNP G Antibody (SR2422) [NBP3-27724] -

Immunohistochemistry-Paraffin: hnRNP G Antibody (SR2422) [NBP3-27724] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Applications for hnRNP G Antibody (SR2422)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP G

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. hnRNP G is a glycoprotein, which was originally identified as an autoantigen from German shepherd dogs with lupus-like syndrome. The gene encoding hnRNP G is located on chromosome Xq26 and is ubiquitously expressed. It contains one RNP-consensus RNA binding domain (RBD) and is related to RBMY, which is involved in spermatogenesis, and hnRNP G-T, which is a testis specific protein. All three proteins interact with Tra2b and therefore, are involved in pre-mRNA splicing.

Alternate Names

Glycoprotein p43, heterogeneous nuclear ribonucleoprotein G, hnRNP G, hnRNP-G, HNRPG, RBMXP1, RBMXRT, RNA binding motif protein, X chromosome, RNA binding motif protein, X-linked, RNA-binding motif protein, X chromosome, RNMX

Gene Symbol

RBMX

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Product Documents for hnRNP G Antibody (SR2422)

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Certificate of Analysis

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Product Specific Notices for hnRNP G Antibody (SR2422)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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