hnRNP H Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31648

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Chicken (96%), Rat (99%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Gel Supershift Assay

Cited:

Gel Supershift Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all hnRNP H Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP H. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%), Zebrafish (87%).

Localization

Nucleus, nucleoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for hnRNP H Antibody - BSA Free

Western Blot: hnRNP H Antibody [NBP1-31648]

Western Blot: hnRNP H Antibody [NBP1-31648]

Western Blot: hnRNP H Antibody [NBP1-31648] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NBP1-31648]

Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NBP1-31648]

Immunocytochemistry/Immunofluorescence: hnRNP H Antibody [NBP1-31648] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP H protein stained by hnRNP H antibody [N1C1] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: hnRNP H Antibody [NBP1-31648]

Immunohistochemistry-Paraffin: hnRNP H Antibody [NBP1-31648]

Immunohistochemistry-Paraffin: hnRNP H Antibody [NBP1-31648] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.
Western Blot: hnRNP H Antibody [NBP1-31648]

Western Blot: hnRNP H Antibody [NBP1-31648]

Western Blot: hnRNP H Antibody [NBP1-31648] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hnRNP H Antibody [NBP1-31648]

Western Blot: hnRNP H Antibody [NBP1-31648]

Western Blot: hnRNP H Antibody [NBP1-31648] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NBP1-31648]

Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NBP1-31648]

Immunocytochemistry/Immunofluorescence: hnRNP H Antibody [NBP1-31648] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP H protein stained by hnRNP H antibody [N1C1] diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:1000. Blue: Hoechst 33342 staining.
hnRNP H Antibody

Western Blot: hnRNP H Antibody [NBP1-31648] -

Western Blot: hnRNP H Antibody [NBP1-31648] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody [N1C1] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for hnRNP H Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Application Notes
Use in Gel super shift assay reported in scientific literature (PMID: 30503283).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: hnRNP H

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype.

Alternate Names

DKFZp686A15170, heterogeneous nuclear ribonucleoprotein H, heterogeneous nuclear ribonucleoprotein H1 (H), hnRNP H, HNRPH, HNRPH1hnRNPH

Gene Symbol

HNRNPH1

Additional hnRNP H Products

Product Documents for hnRNP H Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for hnRNP H Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for hnRNP H Antibody - BSA Free

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Protocols

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