hnRNP L Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03640

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all hnRNP L Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 282-589 of human hnRNP L (NP_001524.2). TWDYTNPNLSGQGDPGSNPNKRQRQPPLLGDHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGRRMGPPVGGHRRGPSRYGPQYGHPPPPPPPPEYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKLNVCVSKQPAIMPGQSYGLEDGSCSYKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPSSVKVFSGKSERSSSGLLEWESKSDALETLGFLNHYQMKNPNGPYPYTLKLCFSTAQHAS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP L Antibody - BSA Free

Western Blot: hnRNP L AntibodyBSA Free [NBP3-03640]

Western Blot: hnRNP L AntibodyBSA Free [NBP3-03640]

Western Blot: hnRNP L Antibody [NBP3-03640] - Analysis of extracts of various cell lines, using hnRNP L antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Immunocytochemistry/ Immunofluorescence: hnRNP L Antibody - BSA Free [NBP3-03640]

Immunocytochemistry/ Immunofluorescence: hnRNP L Antibody - BSA Free [NBP3-03640]

Immunocytochemistry/Immunofluorescence: hnRNP L Antibody [NBP3-03640] - Analysis of 293T cells using hnRNP L antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: hnRNP L Antibody - BSA Free [NBP3-03640]

Immunohistochemistry-Paraffin: hnRNP L Antibody - BSA Free [NBP3-03640]

Immunohistochemistry-Paraffin: hnRNP L Antibody [NBP3-03640] - Immunohistochemistry of paraffin-embedded mouse brain using hnRNP L antibody (NBP3-03640) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: hnRNP L Antibody - BSA Free [NBP3-03640]

Immunohistochemistry-Paraffin: hnRNP L Antibody - BSA Free [NBP3-03640]

Immunohistochemistry-Paraffin: hnRNP L Antibody [NBP3-03640] - Paraffin-embedded rat lung using hnRNP L antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: hnRNP L Antibody - BSA Free [NBP3-03640]

Immunohistochemistry-Paraffin: hnRNP L Antibody - BSA Free [NBP3-03640]

Immunohistochemistry-Paraffin: hnRNP L Antibody [NBP3-03640] - Immunohistochemistry of paraffin-embedded human placenta using hnRNP L antibody (NBP3-03640) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for hnRNP L Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: hnRNP L

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (HNRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with HNRNP complexes and along with other HNRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of HNRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions.Strucutrally, hnRPL contains 2 segments of approximately 80 amino acids each which are weakly related to each other and to the ribonucleoprotein consensus sequence-type RNA-binding domains of other HNRNP and snRNP proteins.

Alternate Names

FLJ35509, heterogeneous nuclear ribonucleoprotein L, hnRNP L, hnRNP-L, HNRPL

Gene Symbol

HNRNPL

Additional hnRNP L Products

Product Documents for hnRNP L Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for hnRNP L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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