hnRNP-R Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89676

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MANQVNGNAVQLKEEEEPMDTSSVTHTEHYKTLIEAGLPQKVAERLDEIFQT

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25338097).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP-R Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: hnRNP-R Antibody [NBP1-89676]

Immunocytochemistry/ Immunofluorescence: hnRNP-R Antibody [NBP1-89676]

Immunocytochemistry/Immunofluorescence: hnRNP-R Antibody [NBP1-89676] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676]

Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676]

Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676] - Staining in human endometrium and pancreas tissues using anti-HNRNPR antibody. Corresponding HNRNPR RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676]

Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676]

Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676] - Staining of human endometrium shows high expression.
Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676]

Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676]

Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676] - Staining of human pancreas shows low expression as expected.
hnRNP-R Antibody - BSA Free Western Blot: hnRNP-R Antibody - BSA Free [NBP1-89676]

Western Blot: hnRNP-R Antibody - BSA Free [NBP1-89676]

Analysis in human cell line HEK 293.

Applications for hnRNP-R Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP-R

The hnRNP-R gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in th

Alternate Names

FLJ25714, heterogeneous nuclear ribonucleoprotein R, hnRNP R, HNRPRhnRNP-R

Gene Symbol

HNRNPR

Additional hnRNP-R Products

Product Documents for hnRNP-R Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for hnRNP-R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for hnRNP-R Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for hnRNP-R Antibody - BSA Free

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  • Q: I am looking for an antibody to recognize mouse hnRNPR I see you have 2 that use almost identical immunogens but recognize proteins of different sizes? NBP1-89676 and NBP1-57158

    A: The differences you are seeing between the NBP1-89676 and NBP1-57158 are most likely due to the sample used in testing. For NBP1-89676, U-251MG and RT4 cell lysates were used in the testing, whereas for NBP1-57158, a 721_B cell lysate was used. It is very possible that these cell lines express different isoforms or different levels of each isoform. Unfortunately, I do not have any data as to which cell line expresses which isoform(s) of this protein.

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