HOXB2 Antibody (PCRP-HOXB2-1F2)
Novus Biologicals | Catalog # NBP3-13937
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-HOXB2-1F2
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Product Specifications
Immunogen
Recombinant fragment (aa114-220) of human HOXB2 (Uniprot: P14652)
Localization
Nucleus, Nucleoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
37.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14063)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for HOXB2 Antibody (PCRP-HOXB2-1F2)
![Immunocytochemistry/ Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]](https://resources.rndsystems.com/images/products/HOXB2-Antibody-PCRP-HOXB2-1F2-Immunocytochemistry-Immunofluorescence-NBP3-13937-img0002.jpg "Immunocytochemistry/ Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]")
Immunocytochemistry/ Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]
Immunocytochemistry/Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HOXB2 Antibody (PCRP-HOXB2-1F2). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).![Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]](https://resources.rndsystems.com/images/products/HOXB2-Antibody-PCRP-HOXB2-1F2-Immunohistochemistry-Paraffin-NBP3-13937-img0004.jpg "Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]")
Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]
Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.![Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]](https://resources.rndsystems.com/images/products/HOXB2-Antibody-PCRP-HOXB2-1F2-Flow-Cytometry-NBP3-13937-img0001.jpg "Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]")
Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]
Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Flow cytometric analysis of PFA-fixed HeLa cells. HOXB2 antibody (PCRP-HOXB2-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).![Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]](https://resources.rndsystems.com/images/products/HOXB2-Antibody-PCRP-HOXB2-1F2-Immunohistochemistry-Paraffin-NBP3-13937-img0003.jpg "Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]")
Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]
Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.![Protein Array: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]](https://resources.rndsystems.com/images/products/HOXB2-Antibody-PCRP-HOXB2-1F2-Protein-Array-NBP3-13937-img0006.jpg "Protein Array: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]")
Protein Array: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]
Protein Array: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HOXB2 Antibody (PCRP-HOXB2-1F2).Applications for HOXB2 Antibody (PCRP-HOXB2-1F2)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: HOXB2
Alternate Names
homeo box 2H, homeo box B2, homeobox B2, Homeobox protein Hox-2.8, Homeobox protein Hox-2H, homeobox protein Hox-B2, HOX2, HOX2HHox-2.8, K8, K8 home protein
Gene Symbol
HOXB2
UniProt
Additional HOXB2 Products
Product Documents for HOXB2 Antibody (PCRP-HOXB2-1F2)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HOXB2 Antibody (PCRP-HOXB2-1F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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