HOXB4 Antibody (3A2F2) - BSA Free

Novus Biologicals | Catalog # NBP2-37257

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunomicroscopy, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3A2F2

Format

BSA Free
View all HOXB4 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human HOXB4 (AA: 16-251) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HOXB4 Antibody (3A2F2) - BSA Free

Western Blot: HOXB4 Antibody (3A2F2)BSA Free [NBP2-37257]

Western Blot: HOXB4 Antibody (3A2F2)BSA Free [NBP2-37257]

Western Blot: HOXB4 Antibody (3A2F2) [NBP2-37257] - Western blot analysis using HOXB4 mAb against HEK293 (1) and HOXB4 (AA: 16-251)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) - BSA Free [NBP2-37257]

Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) - BSA Free [NBP2-37257]

Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) [NBP2-37257] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HOXB4 mouse mAb with DAB staining.
Western Blot: HOXB4 Antibody (3A2F2)BSA Free [NBP2-37257]

Western Blot: HOXB4 Antibody (3A2F2)BSA Free [NBP2-37257]

Western Blot: HOXB4 Antibody (3A2F2) [NBP2-37257] - Western blot analysis using HOXB4 mAb against human HOXB4 (AA: 16-251) recombinant protein. (Expected MW is 51.9 kDa)
Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) - BSA Free [NBP2-37257]

Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) - BSA Free [NBP2-37257]

Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) [NBP2-37257] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HOXB4 mouse mAb with DAB staining.
ELISA: HOXB4 Antibody (3A2F2) - BSA Free [NBP2-37257]

ELISA: HOXB4 Antibody (3A2F2) - BSA Free [NBP2-37257]

ELISA: HOXB4 Antibody (3A2F2) [NBP2-37257] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Immunomicroscopy: HOXB4 Antibody (3A2F2) - BSA Free [NBP2-37257]

Immunomicroscopy: HOXB4 Antibody (3A2F2) - BSA Free [NBP2-37257]

Immunomicroscopy: HOXB4 Antibody (3A2F2) [NBP2-37257] - HeLa cells after 1 minute co-incubation using HoxB4 (7 micro M) and 6His-CM18-PTD4 (10 micro M) in fluorescent microscopy analysis. Prior to immune-labeling, cells were permeabilized and fixed with a primary mouse anti-HOXB4 monoclonal antibody (3A2F2). Scale Bars: 50 micro m. Citation: Del'Guidice T, Lepetit-Stoffaes J-P, Bordeleau L-J, Roberge J, Theberge V, Lauvaux C, et al. (2018) Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells. PLoS ONE 13(4): e0195558. https://doi.org/10.1371/journal.pone.0195558

Applications for HOXB4 Antibody (3A2F2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200-1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HOXB4

Homebox transcription factors (Hox) are encoded by highly conserved developmental genes that are involved embryonic and early hematopoietic development. Specifically, Homeobox B4 (HoxB4) is a transcription factor encoded by HOXB4 gene and it is involved in the developmental regulation of specific positional identities on the anterior-posterior axis of cells. Like all members of HOX family, HoXB4 is abundantly expressed in primitive hematopoietic stem cell (HSC), but then decline with lineage-specific terminal differentiation (1). In HSC, stem cell self-renewal activity is regulated by USF1/2 binding to HoxB4, where binding possibly favors stem cell self-renewal instead of cell differentiation. Jun-B and Fra-1 has been linked as key mediators during cell proliferation and differentiation induced by HoxB4, which leads to increase expression of Cyclin D1 and G1 shortening (2).

Long Name

Homeobox B4

Alternate Names

Hox-2.6, HOX2F

Gene Symbol

HOXB4

Additional HOXB4 Products

Product Documents for HOXB4 Antibody (3A2F2) - BSA Free

Certificate of Analysis

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Product Specific Notices for HOXB4 Antibody (3A2F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HOXB4 Antibody (3A2F2) - BSA Free

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Protocols

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