HOXB5 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04470

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all HOXB5 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 170-269 of human HOXB5 (NP_002138.1). EGQTPQIFPWMRKLHISHDMTGPDGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSLATAGSAFQP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HOXB5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: HOXB5 Antibody - Azide and BSA Free [NBP3-04470]

Immunocytochemistry/ Immunofluorescence: HOXB5 Antibody - Azide and BSA Free [NBP3-04470]

Immunocytochemistry/Immunofluorescence: HOXB5 Antibody [NBP3-04470] - Analysis of U2OS cells using HOxB5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
HOXB5 Antibody - Azide and BSA Free

Western Blot: HOXB5 Antibody [NBP3-04470] -

Western Blot: HOXB5 Antibody [NBP3-04470] - Analysis of lysates from Rat lung, using HOXB5 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.2s.
HOXB5 Antibody - Azide and BSA Free

Western Blot: HOXB5 Antibody [NBP3-04470] -

Western Blot: HOXB5 Antibody [NBP3-04470] - Analysis of various lysates using HOXB5 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.

Applications for HOXB5 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HOXB5

HOXB5 is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in lung and gut development. Increased expression of this gene is associated with a distinct biologic subset of acute myeloid leukemia (AML) and the occurrence of bronchopulmonary sequestration (BPS) and congenital cystic adenomatoid malformation (CCAM) tissue. [provided by RefSeq]

Alternate Names

HHO.C10, homeo box 2A, homeo box B5, homeobox B5, Homeobox protein HHO.C10, Homeobox protein Hox-2A, homeobox protein Hox-B5, Homeobox protein Hu-1, Hox2.1, HOX2AHOX2, HU-1

Gene Symbol

HOXB5

Additional HOXB5 Products

Product Documents for HOXB5 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HOXB5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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