HOXD13 Antibody

Novus Biologicals | Catalog # NBP2-75091

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (%), Mouse (%), Porcine (%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HOXD13 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HGVGLQQNALKSSPH, from the internal region of the protein sequence according to NP_000514.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

34.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HOXD13 Antibody

Western Blot: HOXD13 Antibody [NBP2-75091]

Western Blot: HOXD13 Antibody [NBP2-75091]

Western Blot: HOXD13 Antibody [NBP2-75091] - Staining of Rat Brain lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: HOXD13 Antibody [NBP2-75091]

Immunocytochemistry/ Immunofluorescence: HOXD13 Antibody [NBP2-75091]

Immunocytochemistry/Immunofluorescence: HOXD13 Antibody [NBP2-75091] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP2-75091]

Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP2-75091]

Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP2-75091] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Immunocytochemistry/ Immunofluorescence: HOXD13 Antibody [NBP2-75091]

Immunocytochemistry/ Immunofluorescence: HOXD13 Antibody [NBP2-75091]

Immunocytochemistry/Immunofluorescence: HOXD13 Antibody [NBP2-75091] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP2-75091]

Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP2-75091]

Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP2-75091] - Staining of paraffin embedded Human Prostate with antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for HOXD13 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

TBS (pH 7.3), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HOXD13

HOXD13 belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. Mutations in this particular gene cause synpolydactyly. [provided by RefSeq]

Alternate Names

BDSD, homeo box 4I, homeo box D13, homeobox D13, Homeobox protein Hox-4I, homeobox protein Hox-D13, HOX4IBDE, SPD

Gene Symbol

HOXD13

Additional HOXD13 Products

Product Documents for HOXD13 Antibody

Certificate of Analysis

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Product Specific Notices for HOXD13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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