HP1 alpha Antibody (2H4E9) - BSA Free

Novus Biologicals | Catalog # NBP2-52420

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2H4E9

Format

BSA Free
View all HP1 alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human HP1 alpha (AA: 1-191) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HP1 alpha Antibody (2H4E9) - BSA Free

Western Blot: HP1 alpha Antibody (2H4E9)BSA Free [NBP2-52420]

Western Blot: HP1 alpha Antibody (2H4E9)BSA Free [NBP2-52420]

Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using HP1 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), and A431 (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis of Hela cells using HP1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis of colon tissues using CBX5 mouse mAb with DAB staining.
Western Blot: HP1 alpha Antibody (2H4E9)BSA Free [NBP2-52420]

Western Blot: HP1 alpha Antibody (2H4E9)BSA Free [NBP2-52420]

Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: HP1 alpha Antibody (2H4E9)BSA Free [NBP2-52420]

Western Blot: HP1 alpha Antibody (2H4E9)BSA Free [NBP2-52420]

Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using CBX5 mAb against human CBX5 (AA: 1-191) recombinant protein. (Expected MW is 48.2 kDa)
Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Immunofluorescence analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis of colon cancer tissues using CBX5 mouse mAb with DAB staining.
ELISA: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

ELISA: HP1 alpha Antibody (2H4E9) - BSA Free [NBP2-52420]

ELISA: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for HP1 alpha Antibody (2H4E9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HP1 alpha

Heterochromatin protein-1 (HP1) is a methyl-lysine binding adapter protein that is involved in a variety important functions in the cell nucleus. Localized at heterochromatin sites, HP1 is responsible for gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.

HP1 antibodies are useful tools for research on transcription regulation and epigenetic signaling.

Alternate Names

Antigen p25, chromobox homolog 5, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1-ALPHA, HP1Hs alpha, HP1Hs-alpha

Gene Symbol

CBX5

Additional HP1 alpha Products

Product Documents for HP1 alpha Antibody (2H4E9) - BSA Free

Certificate of Analysis

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Product Specific Notices for HP1 alpha Antibody (2H4E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HP1 alpha Antibody (2H4E9) - BSA Free

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Protocols

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FAQs

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