HPa2 Antibody (DHIC2-2B4) - BSA Free
Novus Biologicals | Catalog # NBP1-18950
![Immunocytochemistry/ Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]](https://resources.rndsystems.com/images/products/HPa2-Antibody-DHIC2-2B4-Immunocytochemistry-Immunofluorescence-NBP1-18950-img0003.jpg "Immunocytochemistry/ Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]")
Key Product Details
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Scientific Data Images for HPa2 Antibody (DHIC2-2B4) - BSA Free
Immunocytochemistry/ Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]
Immunocytochemistry/Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Immunofluorescence on frozen section of human pancreas.![Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]](https://resources.rndsystems.com/images/products/HPa2-Antibody-DHIC2-2B4-Flow-Cytometry-NBP1-18950-img0004.jpg "Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]")
Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]
Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Analysis of enzyme dispersed human pancreas cells.Applications for HPa2 Antibody (DHIC2-2B4) - BSA Free
Flow Cytometry
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Frozen
Western Blot
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
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Background: HPa2
Alternate Names
Additional HPa2 Products
Product Documents for HPa2 Antibody (DHIC2-2B4) - BSA Free
Certificate of Analysis
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Product Specific Notices for HPa2 Antibody (DHIC2-2B4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for HPa2 Antibody (DHIC2-2B4) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for HPa2 Antibody (DHIC2-2B4) - BSA Free
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Q: Does HPa2 cross react with other species? Specifically mouse or monkey?
A:
NBP1-18950 hasn't been tested in mouse or monkey but is predicted to cross react with both. It is not guaranteed for use in these species, but if you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program. Under this program by completing an online review with image, detailing your positive or negative results, you receive a discount voucher for 100% of the purchase price of the reviewed product.
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Q: I would be interested by the Test samples for HPi2 and HPa2 antibodies. I would like to use them for FACS and combine them to dissociate human pancreatic alpha-cells and beta-cells as described in Dorrell C et al Diabetologia 2011. However according to your website, both NBP1-18946 and NBP1-18950 seem to be Mouse IgG1, whereas it is stated in ESM Table 2 of this paper that HPa2 would be an IgM. Could you please explain me this discrepancy ?
A: I can confirm that our HPa2 Ab is IgG1. Our Hpi2 antibody is also IgG1. I checked these in our internal database.
-
Q: Does HPa2 cross react with other species? Specifically mouse or monkey?
A:
NBP1-18950 hasn't been tested in mouse or monkey but is predicted to cross react with both. It is not guaranteed for use in these species, but if you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program. Under this program by completing an online review with image, detailing your positive or negative results, you receive a discount voucher for 100% of the purchase price of the reviewed product.
-
Q: I would be interested by the Test samples for HPi2 and HPa2 antibodies. I would like to use them for FACS and combine them to dissociate human pancreatic alpha-cells and beta-cells as described in Dorrell C et al Diabetologia 2011. However according to your website, both NBP1-18946 and NBP1-18950 seem to be Mouse IgG1, whereas it is stated in ESM Table 2 of this paper that HPa2 would be an IgM. Could you please explain me this discrepancy ?
A: I can confirm that our HPa2 Ab is IgG1. Our Hpi2 antibody is also IgG1. I checked these in our internal database.