HPRT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92006

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HPRT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HPRT Antibody - BSA Free

Western Blot: HPRT Antibody [NBP1-92006]

Western Blot: HPRT Antibody [NBP1-92006]

Western Blot: HPRT Antibody [NBP1-92006] - Analysis in human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: HPRT Antibody [NBP1-92006]

Immunocytochemistry/ Immunofluorescence: HPRT Antibody [NBP1-92006]

Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-92006] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human testis shows very strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human kidney shows strong cytoplasmic positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
HPRT Antibody - BSA Free Western Blot: HPRT Antibody - BSA Free [NBP1-92006]

Western Blot: HPRT Antibody - BSA Free [NBP1-92006]

Analysis in human cell line HEK 293.
HPRT Antibody - BSA Free Immunohistochemistry: HPRT Antibody - BSA Free [NBP1-92006]

Immunohistochemistry: HPRT Antibody - BSA Free [NBP1-92006]

Analysis in human testis and skeletal muscle tissues using NBP1-92006 antibody. Corresponding HPRT1 RNA-seq data are presented for the same tissues.

Applications for HPRT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPRT

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Long Name

Hypoxanthine-Guanine Phosphoribosyltransferase

Alternate Names

HGPRT, HGPRT1, HPRT1

Entrez Gene IDs

3251 (Human)

Gene Symbol

HPRT1

UniProt

P00492

Additional HPRT Products

Product Documents for HPRT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HPRT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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