HRP Antibody (HP-03) - BSA Free

Novus Biologicals | Catalog # NB500-420

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Non-species specific, Plant

Cited:

Bovine

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HP-03

Format

BSA Free
View all HRP Primary Antibodies »

Product Specifications

Immunogen

Horseradish peroxidase (Grade I).

Reactivity Notes

Detects HRP.

Specificity

The antibody HP-03 reacts with horseradish peroxidase. It is suitable for PAP detection system.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HRP Antibody (HP-03) - BSA Free

Western Blot: HRP Antibody (HP-03)BSA Free [NB500-420]

Western Blot: HRP Antibody (HP-03)BSA Free [NB500-420]

Western Blot: HRP Antibody (HP-03) [NB500-420] - Purified horse radish peroxidase sample; non-reduced sample, immunostained by mAb HP-03 and anti-goat anti-mouse IgG (H+L)-HRP conjugate.

Applications for HRP Antibody (HP-03) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:1000
Application Notes
The antibody HP-03 was successfully used for staining of formaldehyde- fixed, Triton X- permeabilized cells transfected with HRP gene.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HRP

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric

Alternate Names

EC 1.11.1.7, Horseradish Peroxidase, HPRC 1, HPRC1, HRPA2, HRPC 2, HRPC2, HRPE5, HRPN, Neutral peroxidase, Peroxidase A2, Peroxidase C1A, Peroxidase C1B, Peroxidase C2, Peroxidase C3, Peroxidase E5, Peroxidase N, PRXC1A, PRXC1B, PRXC2, PRXC3

Additional HRP Products

Product Documents for HRP Antibody (HP-03) - BSA Free

Certificate of Analysis

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Product Specific Notices for HRP Antibody (HP-03) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HRP Antibody (HP-03) - BSA Free

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Protocols

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