HRP Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05282

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep Serum

Format

Azide and BSA Free
View all HRP Primary Antibodies »

Product Specifications

Immunogen

HRP [Horseradish] (Uniprot: Q42517)

Reactivity Notes

Cross reactivity against HRP from other tissues and species may occur but have not been specifically determined.

Specificity

Cross reactivity against HRP from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Sheep

Isotype

Serum

Description

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Peroxidase [Horseradish]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for HRP Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000-1:40000

Western Blot

1:1000-1:4000
Application Notes
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Reconstitution

Reconstitute with 2.0 ml deionized water (or equivalent)

Formulation

Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HRP

Alternate Names

EC 1.11.1.7, Horseradish Peroxidase, HPRC 1, HPRC1, HRPA2, HRPC 2, HRPC2, HRPE5, HRPN, Neutral peroxidase, Peroxidase A2, Peroxidase C1A, Peroxidase C1B, Peroxidase C2, Peroxidase C3, Peroxidase E5, Peroxidase N, PRXC1A, PRXC1B, PRXC2, PRXC3

Additional HRP Products

Product Documents for HRP Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HRP Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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