HSD17B4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33192

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSD17B4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD17B4. The exact sequence is proprietary.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31757939).

Localization

Peroxisome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSD17B4 Antibody - BSA Free

Western Blot: HSD17B4 Antibody [NBP1-33192]

Western Blot: HSD17B4 Antibody [NBP1-33192]

Western Blot: HSD17B4 Antibody [NBP1-33192] - HSD17B4 antibody detects HSD17B4 protein A. 20 ug Rat heart lysate/extract 7.5% SDS-PAGE HSD17B4 antibody dilution: 1:3000
Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody [NBP1-33192]

Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody [NBP1-33192]

Immunocytochemistry/Immunofluorescence: HSD17B4 Antibody [NBP1-33192] - Analysis of methanol-fixed Hep3B, using HSD17B4antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192] - Rat heart. HSD17B4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192] - DLD-1 xenograft, using HSD17B4 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192] - Rat testis. HSD17B4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
HSD17B4 Antibody - BSA Free

Western Blot: HSD17B4 Antibody [NBP1-33192] -

3T3-L1 and differentiated 3T3-L1 (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSD17B4 antibody (NBP1-33192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for HSD17B4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSD17B4

The protein encoded by the HSD17B4 gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathwayfor fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chainand 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidationactivity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present onchromosome 8. (provided by RefSeq)

Alternate Names

12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, 7-alpha, D-bifunctional protein, EDH17B4, hydroxysteroid (17-beta) dehydrogenase 4, MPF-2, peroxisomal, peroxisomal multifunctional enzyme type 2,17-beta-HSD IV, peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV, short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Entrez Gene IDs

3295 (Human); 15488 (Mouse)

Gene Symbol

HSD17B4

UniProt

P51659 (Human)

Additional HSD17B4 Products

Product Documents for HSD17B4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSD17B4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for HSD17B4 Antibody - BSA Free

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Protocols

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