HSF1 [p Ser326] Antibody (SR2246) - BSA Free

Novus Biologicals | Catalog # NBP3-21520

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2246 expressed in HEK293

Format

BSA Free
View all HSF1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-HSF1 [Ser326] (Uniprot #: Q00613 )

Modification

p Ser 326

Specificity

Detects endogenous levels of total phospho-HSF1 [Ser326]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSF1 [p Ser326] Antibody (SR2246) - BSA Free

Western Blot: HSF1 [p Ser 326] Antibody (SR2246) [NBP3-21520] -

Western Blot: HSF1 [p Ser 326] Antibody (SR2246) [NBP3-21520] - Western blot analysis using NBP3-21520 in HeLa cell lysates treated with heat.

Applications for HSF1 [p Ser326] Antibody (SR2246) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSF1

Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Gene Symbol

HSF1

Additional HSF1 Products

Product Documents for HSF1 [p Ser326] Antibody (SR2246) - BSA Free

Certificate of Analysis

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Product Specific Notices for HSF1 [p Ser326] Antibody (SR2246) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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