HSP27 Antibody (G3.1)

Novus Biologicals | Catalog # NBP2-32972

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Chimpanzee, Monkey, Sheep

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # G3.1
View all HSP27 Primary Antibodies »

Product Specifications

Immunogen

Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.

Localization

Predominantly cytoplasmic with some nuclear

Specificity

monoclonal antibody G3.1 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34621)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HSP27 Antibody (G3.1)

Western Blot: HSP27 Antibody (G3.1) [NBP2-32972]

Western Blot: HSP27 Antibody (G3.1) [NBP2-32972]

Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).
Immunocytochemistry/ Immunofluorescence: HSP27 Antibody (G3.1) [NBP2-32972]

Immunocytochemistry/ Immunofluorescence: HSP27 Antibody (G3.1) [NBP2-32972]

Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) [NBP2-32972] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972]

Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972]

Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffin-embedded human Breast Carcinome stained with HSP27 Monoclonal Antibody (G3.1)
Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972]

Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972]

Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972]

Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972]

Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffim-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)
HSP27 Antibody (G3.1)

Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] -

Hsp90 inhibition reduces cell viability in a concentration-dependent manner and increases cytosolic Hsp70 and Hsp27 levels. (A, B) Toxicity assay of B16F10 (A) and LS174T (B) WT and LDH−/− cells treated with NVP-AUY922 (0, 5, 10, 50, 100 nM) for 24 h. (C, D) Representative immunoblot showing intracellular Hsp70, Hsp27 and beta -Actin levels in B16F10 (C) and LS174T (D) cells upon treatment with NVP-AUY922 (100 nM) for 24 h. Quantification of the heat shock protein (HSP) levels are shown in the adjacent bar chart (**p ≤ 0.01, ***p ≤ 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35463341), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for HSP27 Antibody (G3.1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-32972 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

UniProt

P04792

Additional HSP27 Products

Product Documents for HSP27 Antibody (G3.1)

Certificate of Analysis

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Product Specific Notices for HSP27 Antibody (G3.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSP27 Antibody (G3.1)

Customer Reviews for HSP27 Antibody (G3.1) (1)

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  • HSP27 Antibody (G3.1)
    Name: Byron Baron
    Application: Western Blot
    Sample Tested: SW480 (colon)
    Species: Human
    Verified Customer | Posted 12/24/2016
    Marker, SW480 (colon), HT29 (colon), Colo320 (colon) (25ug protein per well). HT29 gave a non-specific set of bands at around 40kDa
    Primary mouse anti HSP27 antibody diluted 1:1000 in TBS. Membrane incubation for 2 hours at RT. Secondary IRDye 800CW goat anti mouse antibody diluted 1:10000 in TBS. Membrane incubation for 1 hour at RT. Detection by Li-Cor Odyssey
    HSP27 Antibody (G3.1) NBP2-32972

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FAQs for HSP27 Antibody (G3.1)

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  • Q: What is the concentration of the existing lot of NBP2-32972-0.1ml

    A: The concentration for product NBP2-32972 is 0.2mg/ml.

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