Hsp47 Antibody (3K3H1)

Novus Biologicals | Catalog # NBP3-15430

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3K3H1 expressed in HEK293
View all HSP47 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 319-418 of human Hsp47 (P50454). KHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hsp47 Antibody (3K3H1)

Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430]

Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430]

Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430] - Western blot analysis of extracts of various cell lines, using Hsp47 Rabbit mAb (NBP3-15430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (3K3H1) [NBP3-15430]

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (3K3H1) [NBP3-15430]

Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (3K3H1) [NBP3-15430] - Immunofluorescence analysis of NIH-3T3 cells using Hsp47 Rabbit mAb (NBP3-15430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Hsp47 Antibody (3K3H1) [NBP3-15430]

Immunohistochemistry-Paraffin: Hsp47 Antibody (3K3H1) [NBP3-15430]

Immunohistochemistry-Paraffin: Hsp47 Antibody (3K3H1) [NBP3-15430] - Immunohistochemistry of paraffin-embedded human colon using Hsp47 Rabbit mAb (NBP3-15430) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Hsp47 Antibody (3K3H1)

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (3K3H1) [Hsp47] -

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (3K3H1) [Hsp47] - Confocal imaging of NIH/3T3 cells using Hsp47 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Hsp47 Antibody (3K3H1)

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (3K3H1) [Hsp47] -

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (3K3H1) [Hsp47] - Confocal imaging of U-2 OS cells using Hsp47 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for Hsp47 Antibody (3K3H1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP47

Hsp47 encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Gene Symbol

SERPINH1

Additional HSP47 Products

Product Documents for Hsp47 Antibody (3K3H1)

Certificate of Analysis

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Product Specific Notices for Hsp47 Antibody (3K3H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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