Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Rabbit, Sheep

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # M16.10A1

Format

BSA Free
View all HSP47 Primary Antibodies »

Product Specifications

Immunogen

Native rat HSP47.

Reactivity Notes

Recognizes Sheep HSP47. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Hsp47 Antibody (M16.10A1) - BSA Free

Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - analysis of Hsp47 in NIH/3T3 cell lysate (20ug/lane) using anti-Hsp47 antibody. 5x10^4 NIH/3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 siRNA the following day. Total protein was harvested 72h after transfection. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Hsp47-Antibody-M16-10A1-Immunocytochemistry-Immunofluorescence-NBP1-97491-img0007.jpg
Immunohistochemistry-Frozen: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Immunohistochemistry-Frozen: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Immunohistochemistry-Frozen: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Hsp47 antibody in guinea pig skin (subdermis). Image from verified customer review.
Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of HSP47, mAb (M16.10A1): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).
Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).
Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).
Simple Western: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Simple Western: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Simple Western: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Simple Western lane view shows a specific band for Hsp47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Hsp47 Antibody (M16.10A1)

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] -

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT & BMP signaling pathways play crucial roles in regeneration. a–d Real-time PCR for WNT & BMP signals, including Wnt7b (a), Wnt10a (b), Lef1 (c), & Bmp2 (d) in response to stretch (day 1 & day 7) & release of stretch (day 9); n = 6 for each group. e Schematic of hair cycle activator & inhibitor in response to strain alteration. f Dual immunostaining for K15 & beta -catenin revealed nuclear staining of beta -catenin in hair follicles at day 9 & day 14 when stretch was released. Scale bar = 50 μm. g Dual immunostaining for K15 & phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar = 50 μm. h, i Immunostaining (h) & confocal microscope (i) revealed co-localization of HSP47 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (h) or 10 μm (i). j, k Immunostaining (j) & confocal microscope (k) revealed co-localization of Perilipin-1 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (j) or 10 μm (k). Statistical significance was determined using ANOVA followed by a Bonferroni post hoc test. Data are presented as means ± SEM. *p < 0.05. **p < 0.01. ***p < 0.001. Source data are provided as a Source Data file. *Autofluorescence of hair shafts in g, h Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30944305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Hsp47 Antibody (M16.10A1)

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] -

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT & BMP signaling pathways play crucial roles in regeneration. a–d Real-time PCR for WNT & BMP signals, including Wnt7b (a), Wnt10a (b), Lef1 (c), & Bmp2 (d) in response to stretch (day 1 & day 7) & release of stretch (day 9); n = 6 for each group. e Schematic of hair cycle activator & inhibitor in response to strain alteration. f Dual immunostaining for K15 & beta -catenin revealed nuclear staining of beta -catenin in hair follicles at day 9 & day 14 when stretch was released. Scale bar = 50 μm. g Dual immunostaining for K15 & phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar = 50 μm. h, i Immunostaining (h) & confocal microscope (i) revealed co-localization of HSP47 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (h) or 10 μm (i). j, k Immunostaining (j) & confocal microscope (k) revealed co-localization of Perilipin-1 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (j) or 10 μm (k). Statistical significance was determined using ANOVA followed by a Bonferroni post hoc test. Data are presented as means ± SEM. *p < 0.05. **p < 0.01. ***p < 0.001. Source data are provided as a Source Data file. *Autofluorescence of hair shafts in g, h Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30944305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Hsp47 Antibody (M16.10A1) - BSA Free

Western Blot: Hsp47 Antibody (M16.10A1) - BSA Free [NBP1-97491] -

Biodistribution and HSP47 silencing activity of LNPs in fibrotic mice.a Ex vivo fluorescence imaging and signal quantification of major organs from PBS, AA-T3A-C12 LNP/Cy5-siRNA or MC3 LNP/Cy5-siRNA treated fibrotic mice (representative dataset from n = 3/group). b Scheme of CCl4 and LNP treatment. Mice received intraperitoneal (i.p.) injections of 20% CCl4 (0.7 μl/g) in corn oil twice a week for 4 weeks. LNPs were intravenously (i.v.) administered at a siRNA dose of 5 μg/mouse twice weekly for 2 weeks. c Body weight changes of mice over time during the experiment (n = 5/group). d Body weight at the end of the experiment (n = 5/group). e IF staining of HSP47 in liver sections (representative dataset from n = 5/group). Arrows indicate central veins. Quantitative analysis was performed using ImageJ software (n = 5/group). Scale bar: 100 μm. f Western blot analysis of HSP47 expression in liver lysates (representative dataset from n = 3/group). GAPDH was used as an internal control. Representative images for two sets of mouse liver samples are shown. Quantitative analysis was performed using ImageJ software (n = 3/group). Data are presented as mean +/- SD. G1, healthy mice; G2, PBS-treated fibrotic mice; G3, AA-T3A-C12/siGFP LNP-treated fibrotic mice; G4, AA-T3A-C12/siHSP47 LNP-treated fibrotic mice; G5, MC3/siHSP47 LNP-treated fibrotic mice. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001. a, d, e one-way ANOVA with Tukey’s correction. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36650129), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Hsp47 Antibody (M16.10A1) - BSA Free

Western Blot: Hsp47 Antibody (M16.10A1) - BSA Free [NBP1-97491] -

AA-T3A-C12 LNP-mediated GFP and HSP47 knockdown in activated fibroblasts.a GFP knockdown using AA-T3A-C12/siGFP LNP (n = 3/group). Activated 3T3-GFP fibroblasts were treated with AA-T3A-C12/siGFP LNP at the indicated dose for 24 or 48 h. b Flow cytometry analysis of GFP expression after AA-T3A-C12/siGFP LNP treatment for 48 h (representative dataset from n = 3/group). c Immunofluorescence (IF) staining of HSP47 in LNP-treated activated 3T3 fibroblasts. Scale bar: 20 μm. d and e Western blot analysis of HSP47 expression in LNP-treated activated 3T3 fibroblasts and primary HSCs (representative dataset from n = 3/group). GAPDH was used as an internal control. Quantitative analysis was performed using ImageJ software. Data are presented as mean +/- SD (n = 3). **p < 0.01; ***p < 0.001. b, d, e one-way ANOVA with Tukey’s correction. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36650129), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Hsp47 Antibody (M16.10A1) - BSA Free

Western Blot: Hsp47 Antibody (M16.10A1) - BSA Free [NBP1-97491] -

AA-T3A-C12 LNP-mediated GFP and HSP47 knockdown in activated fibroblasts.a GFP knockdown using AA-T3A-C12/siGFP LNP (n = 3/group). Activated 3T3-GFP fibroblasts were treated with AA-T3A-C12/siGFP LNP at the indicated dose for 24 or 48 h. b Flow cytometry analysis of GFP expression after AA-T3A-C12/siGFP LNP treatment for 48 h (representative dataset from n = 3/group). c Immunofluorescence (IF) staining of HSP47 in LNP-treated activated 3T3 fibroblasts. Scale bar: 20 μm. d and e Western blot analysis of HSP47 expression in LNP-treated activated 3T3 fibroblasts and primary HSCs (representative dataset from n = 3/group). GAPDH was used as an internal control. Quantitative analysis was performed using ImageJ software. Data are presented as mean +/- SD (n = 3). **p < 0.01; ***p < 0.001. b, d, e one-way ANOVA with Tukey’s correction. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36650129), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Hsp47 Antibody (M16.10A1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

1:10-1:500

Simple Western

1:100

Western Blot

1:1000
Application Notes
This antibody is useful in IHC and detects a band of ~47kDa by WB. Immunoprecipitation, Flow Cytometry and ICC/IF were reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate, separated by Size, antibody dilution of 1:100, apparent MW was 57 kDa.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-97491 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP47

Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion.

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Gene Symbol

SERPINH1

Additional HSP47 Products

Product Documents for Hsp47 Antibody (M16.10A1) - BSA Free

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Product Specific Notices for Hsp47 Antibody (M16.10A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hsp47 Antibody (M16.10A1) - BSA Free

Customer Reviews for Hsp47 Antibody (M16.10A1) - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Shenda Gu
    Application: Western Blot
    Sample Tested: NIH3T3 Whole Cell Lysate, 20ug loaded per lane
    Species: Mouse
    Verified Customer | Posted 01/07/2015
    HSP47 Knockdown
    Hsp47 Antibody (M16.10A1) - BSA Free NBP1-97491
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Guinea pig skin
    Species: Other
    Verified Customer | Posted 03/28/2014
    Hsp47 antibody in guinea pig skin (subdermis)
    Hsp47 Antibody (M16.10A1) - BSA Free NBP1-97491

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