HSP60 Antibody (4B9/89) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]

Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in A2058 Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]

Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in ATDC5 Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]

Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in Hela Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]

Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using a Hsp60 Monoclonal Antibody in human endothelial cells.

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]

Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (Hsp60, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with (left panel) or without (right panel) a Hsp60 monoclonal antibody, at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye.

Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]

Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Cancer biopsies of deparaffinized human Breast carcinoma tissue.

Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]

Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Kidney tissue.

Immunoprecipitation: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]

Immunoprecipitation: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500ug whole cell lysate with 2ug of HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent (HRP) at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Dura.