HSP70/HSPA1A Antibody (BB70) - BSA Free

Novus Biologicals | Catalog # NB110-96427

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila, Fish, Guinea Pig, Hamster, Rabbit, Sheep, Xenopus, Yeast

Cited:

Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # BB70

Format

BSA Free
View all HSP70/HSPA1A Primary Antibodies »

Product Specifications

Immunogen

Chicken HSP70/HSP90 complex

Localization

Cytoplasm

Specificity

Detects approx 72 (HSP) and approx 73kDa (HSC).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP70/HSPA1A Antibody (BB70) - BSA Free

Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.
Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSPA1A cells stained brown. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..
Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.
Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Detection in paraffin embedded liver sections and nuclear sections of rat hepatocytes by staining with BB70. First pictures in series show two hours after exposure to stress, the second shows the control.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Courtesy of: G. Matic, University of Belgrade, Serbia.
Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427]

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSPA1A stained brown. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..
HSP70/HSPA1A Antibody (BB70) - BSA Free

Western Blot: HSP70/HSPA1A Antibody (BB70) - BSA Free [NB110-96427] -

Effects of 7-d dirual HS and ZEN treatment on glycolytic skeletal muscle. (A) After 7 d of HS, relative protein abundance of MDA- and 4-HNE-modified proteins was similar between all groups. (B) Antioxidant enzymes relative protein abundance were assessed via western blotting. Treatment with ZEN decreased protein abundance in GPX1; however, all other antioxidant enzymes were similar between groups (n = 6–7/group). (C) Relative protein abundance of select HSPs following environmental HS and ZEN exposure. Ponceau S Stain (PonS) was used as a loading control. Values represent the mean +/- SEM. Groups were compared using a 2 × 2 ANOVA; a main effect of environment (P < 0.05) and/or a main effect of zearalenone (ZEN) (P < 0.05) is indicated. Similar groups are indicated by the same letter where appropriate, with differences determined by a Newman–Keuls post hoc test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35908787), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for HSP70/HSPA1A Antibody (BB70) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/ml of HSP70/HSC70 Antibody was sufficient for detection of HSP70 and HSC70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reviewed Applications

Read 1 review rated 5 using NB110-96427 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport..

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Entrez Gene IDs

24472 (Rat); 423504 (Chicken)

Gene Symbol

HSPA1B

UniProt

P08106 (Chicken)

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody (BB70) - BSA Free

Certificate of Analysis

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Product Specific Notices for HSP70/HSPA1A Antibody (BB70) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSP70/HSPA1A Antibody (BB70) - BSA Free

Customer Reviews for HSP70/HSPA1A Antibody (BB70) - BSA Free (1)

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  • HSP70/HSPA1A Antibody (BB70)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Skeletal muscle tissue
    Species: Pig
    Verified Customer | Posted 06/30/2017
    Analysis of pig skeletal muscle lysates showing detection of Hsp70 protein. Load: 50 ug protein. Block: 5% milk TBST for 1 hour at RT. Primary Antibody: 1:5000 for o/n 4˚C. Secondary Antibody: Horse Anti-Mouse IgG: HRP for 1 minute at RT.
    HSP70/HSPA1A Antibody (BB70) - BSA Free NB110-96427

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Protocols

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail