HSP90 Antibody (3B6)

Novus Biologicals | Catalog # NB120-5455

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 3B6
View all HSP90 Primary Antibodies »

Product Specifications

Immunogen

Purified mouse HSP90.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

Detects heat shock protein 90 kDa (Hsp 90) from human, mouse and rat tissues. This does not detect chicken Hsp 90.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for HSP90 Antibody (3B6)

Western Blot: HSP90 Antibody (3B6) [NB120-5455]

Western Blot: HSP90 Antibody (3B6) [NB120-5455]

Western Blot: HSP90 Antibody (3B6) [NB120-5455] - Analysis of 50ug of various whole cell lysates and 15ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455]

Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455]

Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455]

Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455]

Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455]

Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455]

Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) an HSP90 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455]

Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455]

Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455]

Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455]

Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455]

Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455]

Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Staining of human colon cancer tissue sections.
Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455]

Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455]

Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455]

Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455]

Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.

Applications for HSP90 Antibody (3B6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

1:20

Immunohistochemistry-Paraffin

1:20

Western Blot

1:500
Application Notes
WB: Detects an approx. 90 kDa protein representing Hsp 90 from MCF7 cell extract. ab5455 does not immunoprecipitate Hsp 90 in any form (free or complexed).

Reviewed Applications

Read 1 review rated 5 using NB120-5455 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites diluted with PBS

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP90

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

Long Name

Heat Shock Protein 90

Alternate Names

FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38

Entrez Gene IDs

3320 (Human); 15519 (Mouse); 299331 (Rat)

Gene Symbol

HSP90AA1

Additional HSP90 Products

Product Documents for HSP90 Antibody (3B6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSP90 Antibody (3B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSP90 Antibody (3B6)

Customer Reviews for HSP90 Antibody (3B6) (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: NIH/3T3 whole cell lysate
    Species: Mouse
    Verified Customer | Posted 06/03/2015
    Western blot for HSP90 in NIH/3T3 cell lysates (30ug per lane) using NB120-5455.
    HSP90 Antibody (3B6) NB120-5455

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail