Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4F10

Format

BSA Free
View all HSP90 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Hsp90 (1-732aa) purified from E. coli (NP_005339).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse HSP90 Antibody (4F10) - BSA Free (NBP1-04301) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HSP90 Antibody (4F10) - BSA Free

Western Blot: HSP90 Antibody (4F10)BSA Free [NBP1-04301]

Western Blot: HSP90 Antibody (4F10)BSA Free [NBP1-04301]

Western Blot: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - The Recombinant Human HSP90 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hsp90 recombinant protein Lane 2. : 293T cell lysate Lane 3. : HeLa cell lysate Lane 4. : HepG2 cell lysate
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (4F10) - BSA Free [NBP1-04301]

Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (4F10) - BSA Free [NBP1-04301]

Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - ICC/IF analysis of HSP90 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HSP90 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunohistochemistry-Paraffin: HSP90 Antibody (4F10) - BSA Free [NBP1-04301]

Immunohistochemistry-Paraffin: HSP90 Antibody (4F10) - BSA Free [NBP1-04301]

Immunohistochemistry-Paraffin: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Flow Cytometry: HSP90 Antibody (4F10) - BSA Free [NBP1-04301]

Flow Cytometry: HSP90 Antibody (4F10) - BSA Free [NBP1-04301]

Flow Cytometry: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - Flow cytometry analysis of HSP90 in HepG2 cells. The cell was stained with NBP1-04301 at 2-5ug for 1x10^6 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Applications for HSP90 Antibody (4F10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:100-1:200

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP90

Hsp90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under non-stress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin.

Long Name

Heat Shock Protein 90

Alternate Names

FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38

Entrez Gene IDs

3320 (Human)

Gene Symbol

HSP90AA1

UniProt

P07900

Additional HSP90 Products

Product Documents for HSP90 Antibody (4F10) - BSA Free

Certificate of Analysis

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Download SDS

Product Specific Notices for HSP90 Antibody (4F10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail