HSP90 Antibody (4F3.E8) - BSA Free
Novus Biologicals | Catalog # NBP2-12878
![Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878]](https://resources.rndsystems.com/images/products/HSP90-Antibody-4F3-E8-Western-Blot-NBP2-12878-img0011.jpg "Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Plant
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Electron Microscopy
Cited:
Electron Microscopy
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 4F3.E8
Format
BSA Free
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Product Specifications
Immunogen
Recombinant Human HSP90 purified from E.coli
Reactivity Notes
Immunogen displays sequence identity for non-tested species: Wheat (Triticum spp).
Localization
Cytoplasm, Melanosome
Specificity
Detects approx 90kDa. This antibody detects both a and B forms of HSP90 equally well.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HSP90 Antibody (4F3.E8) - BSA Free
Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878]
Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.![Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (4F3.E8) [NBP2-12878]](https://resources.rndsystems.com/images/products/HSP90-Antibody-4F3-E8-Immunocytochemistry-Immunofluorescence-NBP2-12878-img0007.jpg "Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (4F3.E8) [NBP2-12878]")
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (4F3.E8) [NBP2-12878]
Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F3.E8) [NBP2-12878] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878]](https://resources.rndsystems.com/images/products/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0014.jpg "Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878]")
Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878]
Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878]](https://resources.rndsystems.com/images/products/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0012.jpg "Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878]")
Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878]
Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878]](https://resources.rndsystems.com/images/products/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0013.jpg "Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878]")
Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878]
Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: muscle tissue. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.Applications for HSP90 Antibody (4F3.E8) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:2000
Application Notes
0.5 ug/ml of HSP90 (total) Antibody was sufficient for detection of HSP90alpha in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.2), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HSP90
Long Name
Heat Shock Protein 90
Alternate Names
FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38
Entrez Gene IDs
3320 (Human)
Gene Symbol
HSP90AA1
UniProt
Additional HSP90 Products
Product Documents for HSP90 Antibody (4F3.E8) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HSP90 Antibody (4F3.E8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for HSP90 Antibody (4F3.E8) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
