HspA1B Antibody (2D11) - Azide and BSA Free

Novus Biologicals | Catalog # H00003304-M05

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D11

Format

Azide and BSA Free
View all HspA1B Primary Antibodies »

Product Specifications

Immunogen

HSPA1B (NP_005336, 531 a.a. ~ 611 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLY

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

HSPA1B - heat shock 70kDa protein 1B, (2D11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HspA1B Antibody (2D11) - Azide and BSA Free

Western Blot: HspA1B Antibody (2D11) [H00003304-M05]

Western Blot: HspA1B Antibody (2D11) [H00003304-M05]

Western Blot: HspA1B Antibody (2D11) [H00003304-M05] - Analysis of HSPA1B expression in NIH/3T3 (Cat # L018V1).
Immunohistochemistry-Paraffin: HspA1B Antibody (2D11) [H00003304-M05]

Immunohistochemistry-Paraffin: HspA1B Antibody (2D11) [H00003304-M05]

Immunohistochemistry-Paraffin: HspA1B Antibody (2D11) [H00003304-M05] - Analysis of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml
Western Blot: HspA1B Antibody (2D11) [H00003304-M05]

Western Blot: HspA1B Antibody (2D11) [H00003304-M05]

Western Blot: HspA1B Antibody (2D11) [H00003304-M05] - Analysis of HSPA1B expression in PC-12 (Cat # L012V1).
Western Blot: HspA1B Antibody (2D11) [H00003304-M05]

Western Blot: HspA1B Antibody (2D11) [H00003304-M05]

Western Blot: HspA1B Antibody (2D11) [H00003304-M05] - Analysis of HSPA1B expression in Hela S3 NE (Cat # L013V3).
Proximity Ligation Assay: HspA1B Antibody (2D11) [H00003304-M05]

Proximity Ligation Assay: HspA1B Antibody (2D11) [H00003304-M05]

Proximity Ligation Assay: HspA1B Antibody (2D11) [H00003304-M05] - Analysis of protein-protein interactions between EGFR and HSPA1B. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-HSPA1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction
ELISA: HspA1B Antibody (2D11) [H00003304-M05]

ELISA: HspA1B Antibody (2D11) [H00003304-M05]

ELISA: HspA1B Antibody (2D11) [H00003304-M05] - Detection limit for recombinant GST tagged HSPA1B is approximately 0.03ng/ml as a capture antibody.

Applications for HspA1B Antibody (2D11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HspA1B

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Alternate Names

FLJ54328, Heat shock 70 kDa protein 1/2, heat shock 70 kDa protein 1A/1B, heat shock 70kD protein 1B, heat shock 70kDa protein 1B, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSP70-1B, HSP70-2, HSPA1, HSPA1A

Entrez Gene IDs

3304 (Human)

Gene Symbol

HSPA1B

UniProt

NP_005336

Additional HspA1B Products

Product Documents for HspA1B Antibody (2D11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HspA1B Antibody (2D11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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