HspA1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16896

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HspA1B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HspA1B. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HspA1B Antibody - BSA Free

Immunohistochemistry-Paraffin: HspA1B Antibody [NBP2-16896]

Immunohistochemistry-Paraffin: HspA1B Antibody [NBP2-16896]

Immunohistochemistry-Paraffin: HspA1B Antibody [NBP2-16896] - Human breast cancer. Hsp70 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
HspA1B Antibody

Immunocytochemistry/ Immunofluorescence: HspA1B Antibody [NBP2-16896] -

Immunocytochemistry/ Immunofluorescence: HspA1B Antibody [NBP2-16896] - HspA1B antibody detects HspA1B protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HspA1B stained by HspA1B antibody (NBP2-16896) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
HspA1B Antibody - BSA Free

Immunohistochemistry-Paraffin: HspA1B Antibody [NBP2-16896] -

Hsp70 antibody detects Hsp70 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Hsp70 stained by Hsp70 antibody (NBP2-16896) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HspA1B Antibody - BSA Free

Immunohistochemistry-Paraffin: HspA1B Antibody [NBP2-16896] -

Hsp70 antibody detects Hsp70 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Hsp70 antibody (NBP2-16896) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HspA1B Antibody - BSA Free

Immunohistochemistry: HspA1B Antibody [NBP2-16896] -

Hsp70 antibody detects Hsp70 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Hsp70 stained by Hsp70 antibody (NBP2-16896) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
HspA1B Antibody - BSA Free

Western Blot: HspA1B Antibody [NBP2-16896] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody (NBP2-16896) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HspA1B Antibody - BSA Free

Immunohistochemistry: HspA1B Antibody [NBP2-16896] -

Hsp70 antibody detects Hsp70 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Hsp70 stained by Hsp70 antibody (NBP2-16896) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Applications for HspA1B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HspA1B

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Alternate Names

FLJ54328, Heat shock 70 kDa protein 1/2, heat shock 70 kDa protein 1A/1B, heat shock 70kD protein 1B, heat shock 70kDa protein 1B, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSP70-1B, HSP70-2, HSPA1, HSPA1A

Gene Symbol

HSPA1B

UniProt

P08107

Additional HspA1B Products

Product Documents for HspA1B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HspA1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for HspA1B Antibody - BSA Free

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Protocols

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FAQs

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