HspA6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32761

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSPA6/HSP70B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HspA6. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HspA6 Antibody - BSA Free

Western Blot: HspA6 Antibody [NBP1-32761]

Western Blot: HspA6 Antibody [NBP1-32761]

Western Blot: HspA6 Antibody [NBP1-32761] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: HspA6 Antibody [NBP1-32761]

Immunocytochemistry/ Immunofluorescence: HspA6 Antibody [NBP1-32761]

Immunocytochemistry/Immunofluorescence: HspA6 Antibody [NBP1-32761] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-32761]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-32761]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-32761] - Paraffin-embedded SG xenograft, using antibody at 1:500 dilution.
HspA6 Antibody

Western Blot: HspA6 Antibody [NBP1-32761] -

Western Blot: HspA6 Antibody [NBP1-32761] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HspA6 antibody [C1C3] (NBP1-32761) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for HspA6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSPA6/HSP70B

HSP70B is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HSP70B and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress. Recombinant human HSP70B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Heat Shock Protein A6

Alternate Names

HSP70B

Gene Symbol

HSPA6

Additional HSPA6/HSP70B Products

Product Documents for HspA6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HspA6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for HspA6 Antibody - BSA Free

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Protocols

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FAQs

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