HT F Homogeneous PARP Inhibition Assay Kit
HT F Homogeneous PARP Inhibition Assay Kit Summary
• Highly sensitive fluorescent assay
• Detect as little as 10% inhibition of PARP Activity
• Inhibitors identified by increase in Fluorescent Signal
• No wash steps
• 96 test size
• Rapid – Assay takes ~ 1.5 hr
For the screening of candidate PARP inhibitors and determination of relative IC50 values.
• 10X Cycling Enzymes
• 2 µM NAD
• 20 mM NAD
• PARP 1 Enzyme
• Activated DNA
• 10X Resazurin
• 10X Buffer
• Stop Solution
• 96-well Black Microplate
For research use only. Not for diagnostic use.
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