Human 20S Immunoproteasome Protein, CF

R&D Systems | Catalog # E-370

R&D Systems
Discontinued Product
E-370 has been discontinued. View all 20S Immunoproteasome products.

Key Product Details

Source

Human PBMCs

Applications

Enzyme Activity
View all 20S Immunoproteasome Proteins and Enzymes »

Product Specifications

Source

Human peripheral blood mononuclear cell-derived 20S Immunoproteasome protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

700 kDa

Activity

The Human 20S Immunoproteasome is able to degrade substrates in an ATP-independent manner. It can be activated chemically with SDS (0.035%) or by the addition of PA28. Reaction conditions will need to be optimized for each specific application. We recommend an initial Human 20S Immunoproteasome concentration of 0.5-5 nM.

Reviewed Applications

Read 1 review rated 3 using E-370 in the following applications:

Formulation, Preparation, and Storage

E-370
Formulation Supplied as a solution in HEPES, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: 20S Immunoproteasome

The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multi-complex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non-identical subunits arranged into four stacked rings (1,2). However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, beta 1, 2, and 5, in the two interior rings of the 20S Proteasome are replaced by three IFN-gamma-inducible catalytic subunits: beta 1i/LMP2, beta 2i/LMP7, and beta 5i/MECL-1 (3). The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha/beta, or the PA28 gamma regulatory complexes (3,4). 20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation (3,5,6). Selective inhibition of 20S Immunoproteasome proteolytic activity using small molecule inhibitors is being examined for therapeutic intervention in cancer and inflammatory diseases (7).  

 This protein has been purified from human peripheral blood mononuclear cells, which have been screened and are negative for hepatitis B surface antigen, antibodies to hepatitis C virus, HIV type 1 antigens, and antibodies to HIV type 1 and 2.

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References

  1. Kim, H.M. et al. (2011) Biochim. Biophys. Acta 1809:67.
  2. Xie, Y. (2010) J. Mol. Cell Biol. 2:308.
  3. Kloetzel, P.M. (2001) Nat. Rev. Mol. Cell Biol. 2:179.
  4. Stadtmueller, B.M. & C.P. Hill (2011) Mol. Cell 41:8.
  5. Cascio, P. et al. (2001) EMBO J. 20:2357.
  6. Ferrington, D.A. & D.S. Gregerson (2012) Prog. Mol. Biol. Transl. Sci. 109:75.
  7. Lee, W. & K.B. Kim (2011) Curr. Top. Med. Chem. 11:2923.

Additional 20S Immunoproteasome Products

Product Documents for Human 20S Immunoproteasome Protein, CF

Certificate of Analysis

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Product Specific Notices for Human 20S Immunoproteasome Protein, CF

For research use only

Citations for Human 20S Immunoproteasome Protein, CF

Customer Reviews for Human 20S Immunoproteasome Protein, CF (1)

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  • Human 20S Immunoproteasome Protein, CF
    Name: TAKASHI SHIMADA
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 05/02/2020
    Ordinate: fluorescent count, Em 345/Ex 445 Em band path: 9 nm Ex band path: 20 nm Horizontal: time [sec] Temp: 37 °C 1. i20S(15 nM)/PA28a(150 nM)/S-300(0.01 uM) 2. i20S(15 nM)/PA28a(150 nM)/S-300(0.1 uM) 3. i20S(15 nM)/PA28a(150 nM)/S-300(1 uM) 4. 20S(15 nM)/PA28a(150 nM)/S-300(0.01 uM) 5. 20S(15 nM)/PA28a(150 nM)/S-300(0.1 uM) 6. 20S(15 nM)/PA28a(150 nM)/S-300(1 uM) The hydrolysis activity of proteasome and immunoproteasome is considered to be lower than that of trypsin. About 15 nM is required in the co-presence of PA28 activator. At that time, the substrate concentration should be about 1 uM for the observation of the saturation level.
    Human 20S Immunoproteasome Protein, CF E-370

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