Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all 4-1BB Ligand/TNFSF9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human 4-1BB Ligand
Arg71-Glu254
Accession # P41273

Specificity

Detects human 4‑1BB Ligand/TNFSF9 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) 4‑1BB Ligand, recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhTNF-alpha, rhTRAIL, and rhTWEAK is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human 4‑1BB Ligand/TNFSF9 Biotinylated Antibody

Detection of 4-1BB Ligand/TNFSF9 antibody in Human Daudi cell line antibody by Flow Cytometry.

Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry.

Human Daudi Burkitt lymphoma cell line was stained with Goat Anti-Human 4-1BB Ligand/TNFSF9 Biotinylated Polyclonal Antibody (Catalog # BAF2295, filled histogram) or control antibody (BAF108, open histogram), followed by PE-conjugated Streptavidin (F0040). Staining was performed usinig our Staining Membrane-associated Proteins protocol.

Applications for Human 4‑1BB Ligand/TNFSF9 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Daudi human Burkitt's lymphoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human 4‑1BB Ligand/TNFSF9 (Catalog # 2295-4L)

Reviewed Applications

Read 1 review rated 5 using BAF2295 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 4-1BB Ligand/TNFSF9

4-1BB ligand (4-1BBL; also CD137L) is a 32 kDa type II transmembrane protein that belongs to the TNF superfamily (TNFSF) molecules (1‑4). The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25 aa N-terminal cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa C-terminal extracellular region (5). The extracellular domain (ECD) of 4-1BBL has a jelly-roll, beta -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55 aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes (2, 3, 6). A 26 kDa soluble form of 4-1BBL is known to occur in humans. Although it is presumably generated by MMP activity, its amino acid size is currently unreported (4). The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF-alpha ) by monocytes (7, 8). 4-1BBL binding to CD137/4-1BB produces a number of effects. It seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN-gamma in CD4+ cells, and IFN-gamma in CD8+ cells (9, 10).

References

  1. Kwon, B. et al. (2003) Exp. Mol. Med. 35:8.
  2. Salih, H.R. et al. (2002) Int. J. Clin. Pharmacol. Ther. 40:348.
  3. Vinay, D.S. and B.S. Kwon (1998) Semin. Immunol. 1:481.
  4. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
  5. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  6. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
  7. Michel, J. et al. (1999) Immunology 98:42.
  8. Langstein, J. et al. (1998) J. Immunol. 160:2488.
  9. Wen, T. et al. (2002) J. Immunol. 168:4897.
  10. Dawicki, W. and T.H. Watts (2004) Eur. J. Immunol. 34:743.

Alternate Names

41BB Ligand, CD137L, TNFSF9

Entrez Gene IDs

8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)

Gene Symbol

TNFSF9

UniProt

P41273

Additional 4-1BB Ligand/TNFSF9 Products

Product Documents for Human 4‑1BB Ligand/TNFSF9 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 4‑1BB Ligand/TNFSF9 Biotinylated Antibody

For research use only

Citations for Human 4‑1BB Ligand/TNFSF9 Biotinylated Antibody

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  • Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: HT1080 human fibrosarcoma cell line
    Species: Human
    Verified Customer | Posted 03/09/2023
    Human 4‑1BB Ligand/TNFSF9 Biotinylated Antibody BAF2295

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